Mouse anti-Human TCR alpha/beta Monoclonal Antibody

Anti-Hu TCR alpha/beta PE-Cy5

Cat. Num. AAA1802233

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with tandem dye PE-Cy5 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: TCR alpha/beta; Monoclonal Antibody; Anti-Hu TCR alpha/beta PE-Cy5; TCRA/B; anti-TCR alpha/beta antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: IP26
• Specificity: The mouse monoclonal antibody IP26 recognizes a monomorphic extracellular determinant of TCR alpha/beta, the dominant subtype of T cell receptor expressed in human peripheral blood.
• Purity/Purification: The purified antibody is conjugated with tandem dye PE-Cy5 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-TCR alpha/beta antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Conjugation: PE-Cy5
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR alpha/beta (IP26) PE-Cy™5 antibody (4 ul reagent / 100 ul of peripheral whole blood).)

Flow Cytometry (FC/FACS)

(Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human TCR alpha/beta (IP26) PE-Cy™5 antibody (4 ul reagent / 100 ul of peripheral whole blood) and anti-human CD3 (UCHT1) APC antibody (10 ul reagent / 100 ul of peripheral whole blood).)

Flow Cytometry (FC/FACS)

(Separation of human TCR alpha/beta positive lymphocytes (red-filled) from TCR alpha/beta negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human TCR alpha/beta (IP26) PE-Cy™5 antibody (4 ul reagent / 100 ul of peripheral whole blood).)

Related Product Information for anti-TCR alpha/beta antibody

The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form Functional Application (FUNC)tional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.

References

*Kuttruff S, Koch S, Kelp A, Pawelec G, Rammensee HG, Steinle A: NKp80 defines and stimulates a reactive subset of CD8 T cells. Blood. 2009 Jan 8;113(2):358-69., URL: http://www.ncbi.nlm.nih.gov/pubmed/18922855 Sulliv
*von Boehmer H: T cell development and selection in the thymus. Bone Marrow Transplant. 1992;9 Suppl 1:46-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/1324045ordina,*Levelt CN, Wang B, Ehrfeld A, Terhorst C, Eichmann K: Regulation of T cell receptor (TCR)-beta locus allelic exclusion and initiation of TCR-alpha locus rearrangement in immature thymocytes by signaling through the CD3 complex. Eur J Immunol. 1995 May;25(5):1257-61., URL: https://www.ncbi.nlm.nih.gov/pubmed/7774628ordina,*Michie AM, Zúñiga-Pflücker JC: Regulation of thymocyte differentiation: pre-TCR signals and beta-selection. Semin Immunol. 2002 Oct;14(5):311-23., URL: https://www.ncbi.nlm.nih.gov/pubmed/12220932ordin,*Croxford AL, Akilli-Ozturk O, Rieux-Laucat F, Förster I, Waisman A, Buch T: MHC-restricted T cell receptor signaling is required for alpha beta TCR replacement of the pre T cell receptor. Eur J Immunol. 2008 Feb;38(2):391-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/18203137ordin