Mouse CD3 Monoclonal Antibody | anti-CD3 antibody

Anti-Hu CD3 Purified

• Gene Names: CD3E; T3E; TCRE; IMD18
• Reactivity: Non-human primates, Human
• Applications: Flow Cytometry, Functional Assay, Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Purified by protein-A affinity chromatography
• Synonyms: CD3; Monoclonal Antibody; Anti-Hu CD3 Purified; CD3E; T3E; TCRE; CD3 antigen; epsilon polypeptide; anti-CD3 antibody

• Host: Mouse
• Reactivity: Non-human primates, Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone Number: SK7
• Specificity: The mouse monoclonal antibody SK7 recognizes an extracellular epitope on the CD3 antigen on mature human T cells. This antibody reacts with the epsilon chain of the CD3 complex. The monoclonal antibodies SK7 and UCHT1 recognize overlapping epitopes.
• Purity/Purification: Purified by protein-A affinity chromatography
• Form/Format: Purified. Phosphate buffered saline (PBS) solution with 15mM sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD3 antibody: Flow Cytometry (FC/FACS) (QC tested), Western Blot (WB), Immunohistochemistry-Frozen (IHC-F), Immunocytochemistry (ICC)
• Application Notes: FC/FACS: Recommended dilution: 1-4ug/ml; Immunohistochemistry: Recommended dilution: 5-10ug/ml
• Immunogen: Human thymocytes
• Preparation and Storage: Store at 2-8 degree C. Do not freeze. Do not use after expiration date stamped on the label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD3 (SK7) purified antibody (concentration in sample 4 ug/ml) GAM APC.)

Flow Cytometry (FC/FACS)

(Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD3 (SK7) purified antibody (concentration in sample 4 ug/ml) GAM APC.)

Related Product Information for anti-CD3 antibody

CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

References

*Qiao D, Li L, Guo J, Lao S, Zhang X, Zhang J, Wu C: Mycobacterium tuberculosis culture filtrate protein 10-specific effector/memory CD4+ and CD8+ T cells in tubercular pleural fluid, with biased usage of T cell receptor Vbeta chains. Infect Immun. 2011 Aug;79(8):3358-65., URL: https://www.ncbi.nlm.nih.gov/pubmed/term=qiao+d+g,*Wood GS, Burns BF, Dorfman RF, Warnke RA: The immunohistology of non-T cells in the acquired immunodeficiency syndrome. Am J Pathol. 1985 Sep;120(3):371-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/term=am+j+path,*Li B, Wang H, Dai J, Ji J, Qian W, Zhang D, Hou S, Guo Y: Construction and characterization of a humanized anti-human CD3 monoclonal antibody 12F6 with effective immunoregulation Functional Application (FUNC)tions. Immunology. 2005 Dec;116(4):487-98., URL: http://www.ncbi.nlm.nih.gov/pubmed/term=li+b+wang,*Recio MJ, Moreno-Pelayo MA, Kiliç SS, Guardo AC, Sanal O, Allende LM, Pérez-Flores V, Mencía A, Modamio-Hoybjor S, Seoane E, Regueiro JR: Differential biological role of CD3 chains revealed by human immunodeficiencies. J Immunol. 2007 Feb 15;178(4):2556-64., URL: http://www.ncbi.nlm.nih.gov/pubmed/17277165,*Guttman-Yassky E, Vugmeyster Y, Lowes MA, Chamian F, Kikuchi T, Kagen M, Gilleaudeau P, Lee E, Hunte B, Howell K, Dummer W, Bodary SC, Krueger JG: Blockade of CD11a by efalizumab in psoriasis patients induces a unique state of T-cell hyporesponsiveness. J Invest Dermatol. 2008 May;128(5):1182-91., URL: http://www.ncbi.nlm.nih.gov/pubmed/18239614
*Huang Y, Wange RL: T cell receptor signaling: beyond complex complexes. J Biol Chem. 2004 Jul 9;279(28):28827-30., URL: http://www.ncbi.nlm.nih.gov/sites/entrezDb=pubmed,*Alarcón B, Swamy M, van Santen HM, Schamel WW: T-cell antigen-receptor stoichiometry: pre-clustering for sensitivity. EMBO Rep. 2006 May;7(5):490-5., URL: http://www.ncbi.nlm.nih.gov/sites/entrezDb=pubmed,*Kuhns MS, Davis MM, Garcia KC: Deconstructing the form and Functional Application (FUNC)tion of the TCR/CD3 complex. Immunity. 2006 Feb;24(2):133-9., URL: http://www.ncbi.nlm.nih.gov/sites/entrezDb=pubmed

NCBI and Uniprot Product Information

• NCBI GI #: 1345708
• NCBI GeneID: 916
• UniProt Accession #: P07766; A8K997
• Molecular Weight: 207
• NCBI Official Full Name: T-cell surface glycoprotein CD3 epsilon chain
• NCBI Official Synonym Full Names: CD3e molecule, epsilon (CD3-TCR complex)
• NCBI Official Symbol: CD3E
• NCBI Official Synonym Symbols: T3E; TCRE; IMD18
• NCBI Protein Information: T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell antigen receptor complex, epsilon subunit of T3
• UniProt Protein Name: T-cell surface glycoprotein CD3 epsilon chain
• Protein Family: CD302 antigen
• UniProt Gene Name: CD3E
• UniProt Synonym Gene Names: T3E
• UniProt Entry Name: CD3E_HUMAN

NCBI Description

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

Uniprot Description

CD3E: a T cell surface glycoprotein that is a component of the T cell antigen receptor. The recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. Contains 1 immunoglobulin-like domain and 1 ITAM domain.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: T cell receptor complex; integral to plasma membrane; plasma membrane; immunological synapse; alpha-beta T cell receptor complex; intercellular junction; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; receptor signaling complex scaffold activity; protein heterodimerization activity; receptor signaling protein activity; T cell receptor binding; SH3 domain binding; protein kinase binding

Biological Process: regulation of immune response; T cell activation; positive regulation of interleukin-2 biosynthetic process; signal complex assembly; positive regulation of calcium-mediated signaling; negative thymic T cell selection; T cell receptor signaling pathway; positive regulation of interleukin-4 production; regulation of apoptosis; G-protein coupled receptor protein signaling pathway; positive regulation of interferon-gamma production; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; negative regulation of smoothened signaling pathway; T cell costimulation; protein complex assembly; positive regulation of alpha-beta T cell proliferation; positive regulation of T cell proliferation; positive regulation of T cell anergy; transmembrane receptor protein tyrosine kinase signaling pathway; response to nutrient

Disease: Immunodeficiency 18