Flow Cytometry (FC/FACS)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR gamma/delta (11F2) purified antibody (concentration in sample 1,7ug/ml, GAM APC).)
Mouse anti-Human TCR gamma/delta Antibody | anti-TCRgamma/delta antibody
Anti-Hu TCR gamma/delta Purified
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Immunoprecipitation, Immunohistochemistry
Purity
Purified by protein-A affinity chromatography.
Synonyms
TCR gamma/delta; Anti-Hu TCR gamma/delta Purified; TCRG/D; anti-TCRgamma/delta antibody
Host
Mouse
Reactivity
Human
Isotype
Mouse IgG1
Clone Number
11F2
Specificity
The mouse monoclonal antibody 11F2 recognizes an extracellular epitope in all molecular forms of the TCR gamma/delta.
Purity/Purification
Purified by protein-A affinity chromatography.
Form/Format
Phosphate buffered saline (PBS), pH7.4, 15mM sodium azide
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-TCRgamma/delta antibody
Flow Cytometry (FC/FACS)(QC tested), ELISA (EIA), Immunoprecipitation (IP), Immunohistochemistry-Frozen (IHC-F)
Application Notes
FC/FACS: Recommended dilution: 1-4ug/ml.
Immunogen
Purified CD3 antigen complex
Preparation and Storage
Store at 2-8 degree C. Do not freeze.
Flow Cytometry (FC/FACS)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR gamma/delta (11F2) purified antibody (concentration in sample 1,7ug/ml, GAM APC).)
Flow Cytometry (FC/FACS)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR gamma/delta (11F2) purified antibody (concentration in sample 1,7ug/ml, GAM APC).)
Flow Cytometry (FC/FACS)
(Separation of TCR gamma/delta positive lymphocytes (red-filled) from human TCR gamma/delta negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human TCR gamma/delta (11F2) purified antibody (concentration in sample 1,7ug/ml, GAM APC).)
Flow Cytometry (FC/FACS)
(Separation of TCR gamma/delta positive lymphocytes (red-filled) from human TCR gamma/delta negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human TCR gamma/delta (11F2) purified antibody (concentration in sample 1,7ug/ml, GAM APC).)
Related Product Information for anti-TCRgamma/delta antibody
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lympHand secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
References
Ichinohasama R, Miura I, Takahashi T, Yaginuma Y, Myers J, DeCoteau JF, Yee C, Kadin ME, Mori S, Sawai T: Peripheral CD4+ CD8- gammadelta T cell lymphoma: a case report with multiparameter analyses. Hum Pathol. 1996 Dec;27(12):1370-7., URL: https://pubmed.ncbi.nlm.nih.gov/8958314/
Futagbi G, Welbeck JE, Tetteh JK, Hviid L, Akanmori BD: Selective activation of TCR-gammadelta+ cells in endemic Burkitt's lymphoma. Malar J. 2007 May 23;6:69., URL: https://pubmed.ncbi.nlm.nih.gov/17521425/
Bertaina A, Zorzoli A, Petretto A, Barbarito G, Inglese E, Merli P, Lavarello C, Brescia LP, De Angelis B, Tripodi G, Moretta L, Locatelli F, Airoldi I: Zoledronic acid boosts gammadelta T-cell activity in children receiving alphabeta + T and CD19 + cell-depleted grafts from an HLA-haplo-identical donor. Oncoimmunology. 2016 Sep 27;6(2):e1216291., URL: https://pubmed.ncbi.nlm.nih.gov/28344861/
Papadopoulou M, Tieppo P, McGovern N, Gosselin F, Chan JK, Goetgeluk G, Dauby N, Cogan A, Donner C, Ginhoux F, Vandekerckhove B, Vermijlen D: TCR sequencing reveals the distinct development of fetal and adult human Vgamma9Vdelta2 T cells. J Immunol. 2019 Sep 15;203(6):1468-1479., URL: https://pubmed.ncbi.nlm.nih.gov/31413106/,
Futagbi G, Welbeck JE, Tetteh JK, Hviid L, Akanmori BD: Selective activation of TCR-gammadelta+ cells in endemic Burkitt's lymphoma. Malar J. 2007 May 23;6:69., URL: https://pubmed.ncbi.nlm.nih.gov/17521425/
Bertaina A, Zorzoli A, Petretto A, Barbarito G, Inglese E, Merli P, Lavarello C, Brescia LP, De Angelis B, Tripodi G, Moretta L, Locatelli F, Airoldi I: Zoledronic acid boosts gammadelta T-cell activity in children receiving alphabeta + T and CD19 + cell-depleted grafts from an HLA-haplo-identical donor. Oncoimmunology. 2016 Sep 27;6(2):e1216291., URL: https://pubmed.ncbi.nlm.nih.gov/28344861/
Papadopoulou M, Tieppo P, McGovern N, Gosselin F, Chan JK, Goetgeluk G, Dauby N, Cogan A, Donner C, Ginhoux F, Vandekerckhove B, Vermijlen D: TCR sequencing reveals the distinct development of fetal and adult human Vgamma9Vdelta2 T cells. J Immunol. 2019 Sep 15;203(6):1468-1479., URL: https://pubmed.ncbi.nlm.nih.gov/31413106/,
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Product Notes
The TCRgamma/delta (Catalog #AAA1802258) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu TCR gamma/delta Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TCR gamma/delta can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS)(QC tested), ELISA (EIA), Immunoprecipitation (IP), Immunohistochemistry-Frozen (IHC-F). FC/FACS: Recommended dilution: 1-4ug/ml. Researchers should empirically determine the suitability of the TCRgamma/delta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCR gamma/delta, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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