Mouse anti-Rat Synaptojanin 1 Monoclonal Antibody | anti-SYNJ1 antibody

Anti-Synaptojanin I

Cat. Num. AAA500032

• Reactivity: Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein G purified (Protein G purified culture supernatant)
• Synonyms: Synaptojanin 1; Monoclonal Antibody; Anti-Synaptojanin I; anti-SYNJ1 antibody

• Host: Mouse
• Reactivity: Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5H1
• Specificity: Specific for the ~145k synaptojanin I protein in Western blots of rat brain extracts. Immunolabeling blocked by preadsorption of antibody with the protein used to generate the antibody.
• Purity/Purification: Protein G purified (Protein G purified culture supernatant)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 1292

• Applicable Applications for anti-SYNJ1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000; IHC: 1:100
• Antigen: Recombinant protein corresponding to amino acid residues from the C-terminal region of rat synaptojanin 1.
• Immunogen Information: Recombinant protein from the C-terminal region of rat synaptojanin 1.
• Immunogen Species: Rat
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. Does not cross react with mouse.
• Biological Significance: Synaptojanin is a phosphatidylinositol phosphatase involved in clathrin-mediated endocytosis of synaptic vesicles. Synaptojanin 1 has two alternatively spliced isoforms; one that is ~ 145 kDA and is exclusively expressed in neurons, and the other ~ 170 kDa which is expressed in non-neuronal, peripheral tissues (Ramjaun AR & McPherson PS, 1996). The gene which encodes Synaptojanin 1, SYNJ1, has been mapped to chromosome 21 thus making it a candidate for involvement in Down's syndrome (DS). It has recently been demonstrated that Ts65Dn mice (the most commonly used model of DS) have altered phosphatidylinositol-4,5-bisphosphate metabolism. This defect is rescued by restoring SYNJ1 to disomy in the Ts65Dn mice (Voronov SV et al., 2008).
• Preparation and Storage: For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year

Testing Data

Related Product Information for anti-SYNJ1 antibody

Purified mouse monoclonal antibody

References

• Tissue-specific alternative splicing generates two synaptojanin isoforms with differential membrane binding properties (1996). Ramjaun AR, McPherson PS. J Biol Chem. Oct 4;271(40):24856-61.
• Synaptojanin 1-linked phosphoinositide dyshomeostasis and cognitive deficits in mouse models of Down's syndrome (2008).Voronov SV, Frere SG, Giovedi S, Pollina EA, Borel C, Zhang H, Schmidt C, Akeson EC, Wenk MR, Cimasoni L, Arancio O, Davisson MT, Antonarakis SE, Gardiner K, De Camilli P, Di Paolo G. Proc Natl Acad Sci U S A. Jul 8;105(27):9415-20.

NCBI and Uniprot Product Information

• NCBI GI #: 209870039
• NCBI GeneID: 85238
• NCBI Accession #: NP_445928.2
• NCBI GenBank Nucleotide #: NM_053476.2
• UniProt Accession #: Q62910; O89092; Q62911; Q810Z8
• Molecular Weight: 145
• NCBI Official Full Name: synaptojanin-1
• NCBI Official Synonym Full Names: synaptojanin 1
• NCBI Official Symbol: Synj1
• NCBI Protein Information: synaptojanin-1; synaptic inositol 1,4,5-trisphosphate 5-phosphatase 1; synaptic inositol-1,4,5-trisphosphate 5-phosphatase 1
• UniProt Protein Name: Synaptojanin-1
• Protein Family: Synaptojanin
• UniProt Gene Name: Synj1
• UniProt Entry Name: SYNJ1_RAT

NCBI Description

has inositol 5-phosphatase activity; interacts with dynamin to play a role in synaptic vesicle recycling [RGD, Feb 2006]

Uniprot Description

Function: Inositol 5-phosphatase which has a role in clathrin-mediated endocytosis.

Catalytic activity: 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate.

Subunit structure: Interacts with ASH/GRB2. Interacts with PACSIN1, PACSIN2 and PACSIN3

By similarity. Binds AMPH, SH3GL1, SH3GL2 and SH3GL3. Interacts with MYO1E (via SH3 domain). Ref.4 Ref.5

Subcellular location: Cytoplasm

By similarity.

Tissue specificity: Isoform 1 is found in neonatal brain, and in a wide variety of adult non-neuronal tissues. Isoform 2 is expressed predominantly in the neurons, but is also found in all other tissues at much lower levels. Isoform 1 and isoform 2 are detected in the lung and heart. Isoform 1 is expressed at higher levels than isoform 2 in the testis and liver and both isoforms are not detected in the skeletal muscle. Isoform 3 with the 16-amino-acid insert is only found in the brain while isoform 3 without the 16-amino-acid insert is found in the lung.

Developmental stage: At embryonic day 12 (E12) only isoform 1 is seen while at E16 and E18 isoform 1 and isoform 2 are seen. In the adult brain expression of isoform 2 increases dramatically as compared with its expression in embryonic brain where as isoform 1 decreases to undetectable levels.

Domain: Binds to EPS15 (a clathrin coat-associated protein) via a C-terminal domain containing three Asn-Pro-Phe (NPF) repeats

By similarity.The C-terminal proline-rich region mediates binding to a variety of SH3 domain-containing proteins including AMPH, SH3GL1, SH3GL2, SH3GL3 and GRB2.Splicing of the SAC1 domain does not alter the catalytic activity of synaptojanin 1.

Sequence similarities: Belongs to the synaptojanin family.In the central section; belongs to the inositol 1,4,5-trisphosphate 5-phosphatase family.Contains 1 RRM (RNA recognition motif) domain.Contains 1 SAC domain.