Mouse Syntaxin Monoclonal Antibody | anti-STX1A antibody

Anti-Syntaxin 1A

Cat. Num. AAA500030

• Gene Names: STX1A; STX1; HPC-1; P35-1; SYN1A
• Reactivity: Human, rat, pig, hamster
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein G purified (Protein G purified culture supernatant.)
• Synonyms: Syntaxin; Monoclonal Antibody; Anti-Syntaxin 1A; anti-STX1A antibody

• Host: Mouse
• Reactivity: Human, rat, pig, hamster
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: SP8
• Specificity: The antibody is specific for the ~ 36 kDa syntaxin protein in Western blots of rat brain lysate. The antibody has also been used for immunohistochemistry on formalin fixed, vibratome sections. Does not work on paraffin sections.
• Purity/Purification: Protein G purified (Protein G purified culture supernatant.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 251

• Applicable Applications for anti-STX1A antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1,000; IHC: 1:100
• Antigen: Synaptic immunoprecipitate (crude) from human brain.
• Immunogen Information: Synaptic immunoprecipitate (crude) from human brain
• Immunogen Species: Human
• Species Reactivity Note: The antibody has been directly tested for reactivity in human, rat, pig, and hamster.
• Biological Significance: Syntaxins are a family of proteins involved in the docking of synaptic vesicles with the plasma membrane. Syntaxin, along with synaptobrevin and SNAP-25, forms the SNARE complex which mediates synaptic vesicle fusion and exocytosis. Binding of Munc18-1 to the N-terminus of syntaxin 1 has been demonstrated to be essential for exocytic membrane fusion (Khvotchev et al., 2007). Decreased levels of phosphorylated syntaxin 1 in the prefrontal cortex have recently been correlated with schizophrenia (Castillo et al., 2010).
• Preparation and Storage: Store at -20 degree C; stable for at least one year.

Testing Data

Related Product Information for anti-STX1A antibody

Mouse monoclonal antibody

References

• Khvotchev M, Dulubova I, Sun J, Dai H, Rizo J, Südhof TC (2007) Dual modes of Munc18-1/SNARE interactions are coupled by functionally critical binding to syntaxin-1 N terminus. J Neurosci. 2007 Nov 7;27(45):12147-55
• Castillo MA, Ghose S, Tamminga CA, Ulery-Reynolds PG (2010) Deficits in syntaxin 1 phosphorylation in schizophrenia prefrontal cortex. Biol Psychiatry. 67(3):208-16
• Honer, W. G., Hu, L. & Davies, P. Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex. Brain Research 609, 9-20 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 259906442
• NCBI GeneID: 6804
• NCBI Accession #: NP_001159375.1
• NCBI GenBank Nucleotide #: NM_001165903.1
• UniProt Accession #: Q16623; O15447; O15448; Q12936; Q75MD9; Q9BPZ6
• Molecular Weight: 36
• NCBI Official Full Name: syntaxin-1A isoform 2
• NCBI Official Synonym Full Names: syntaxin 1A (brain)
• NCBI Official Symbol: STX1A
• NCBI Official Synonym Symbols: STX1; HPC-1; P35-1; SYN1A
• NCBI Protein Information: syntaxin-1A; neuron-specific antigen HPC-1
• UniProt Protein Name: Syntaxin-1A
• Protein Family: Syntaxin
• UniProt Gene Name: STX1A
• UniProt Synonym Gene Names: STX1
• UniProt Entry Name: STX1A_HUMAN

NCBI Description

This gene encodes a member of the syntaxin superfamily. Syntaxins are nervous system-specific proteins implicated in the docking of synaptic vesicles with the presynaptic plasma membrane. Syntaxins possess a single C-terminal transmembrane domain, a SNARE [Soluble NSF (N-ethylmaleimide-sensitive fusion protein)-Attachment protein REceptor] domain (known as H3), and an N-terminal regulatory domain (Habc). Syntaxins bind synaptotagmin in a calcium-dependent fashion and interact with voltage dependent calcium and potassium channels via the C-terminal H3 domain. This gene product is a key molecule in ion channel regulation and synaptic exocytosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

Function: Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca2+-regulation of exocytosis acrosomal reaction in sperm.

Subunit structure: Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds to CPLX1. Binds SYTL4 and STXBP6. Found in a ternary complex with STX1A and SNAP25. Interacts with OTOF and LGI3. Interacts with SLC6A4. Interacts with SYT6 and SYT8; the interaction is Ca2+-dependent

By similarity. Found in a complex with VAMP8 and SNAP23. Interacts with VAPA and SYBU. Interacts with STXBP1 and DAPK1. Ref.10 Ref.11 Ref.12

Subcellular location: Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Single-pass type IV membrane protein

By similarity. Cell junction › synapse › synaptosome

By similarity. Isoform 2: Secreted

Probable.

Tissue specificity: Isoform 1 is highly expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain. Ref.9

Post-translational modification: Phosphorylated by CK2

By similarity. Phosphorylation at Ser-188 by DAPK1 significantly decreases its interaction with STXBP1. Ref.11

Involvement in disease: STX1A is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.

Sequence similarities: Belongs to the syntaxin family.Contains 1 t-SNARE coiled-coil homology domain.

Sequence caution: The sequence AAA20940.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.