Rat Sphingosine Kinase 1 Monoclonal Antibody | anti-SPHK1 antibody

Sphingosine Kinase 1 (C-terminal region) M540 Antibody

Cat. Num. AAA474594

• Gene Names: Sphk1; Sk1; Spk1; 1110006G24Rik
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, ELISA, Immunocytochemistry
• Synonyms: Sphingosine Kinase 1; Monoclonal Antibody; Sphingosine Kinase 1 (C-terminal region) M540 Antibody; Sphingosine Kinase 1 (C-terminal region); SPHK1 Cterm1; anti-SPHK1 antibody

• Host: Rat
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Clone Number: M540
• Specificity: This antibody was affinity purified using SK1 (C-terminal Region) peptide. The purified antibody detects 42kDa* full-length recombinant human and mouse SK1 proteins, as well as detects 42, 50, and 60kDa forms of SK1 in human HeLa cells.
• Form/Format: Rat monoclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-SPHK1 antibody: Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
• Application Notes: ELISA: 1:1000; ICC: 1:200; WB: 1:250
• Immunogen: Clone M540 was generated from a SK1 synthetic peptide (coupled to carrier) corresponding to amino acids in the C-terminal region of mouse SK1. This sequence has high homology to human and rat SK1, and is not homologous to sequences in SK2.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot image of human Hela cells (lanes 1 & 2) and recombinant his-tagged human SK1 protein (lanes 3 & 4). The blots were probed with rat monoclonal anti-SK1 (C-terminal Region) at 1:250 (lanes 1 & 3) and 1:1000 (lanes 2 & 4).)

Immunocytochemistry (ICC)

(Immunocytochemical labeling of sphingosine kinase 1 (SPHK1) in paraformaldehyde fixed and NP-40 permeabilized A431 cells. The cells were labeled with rat monoclonal anti-SPHK1 (SM5401). The antibody was detected using goat anti-rat DyLight 594.)

Related Product Information for anti-SPHK1 antibody

Sphingolipids are metabolized into bioactive products that include ceramide, sphingosine, and sphingosine-1-phosphate (S1P). Sphingosine Kinase (SK) catalyzes the phosphorylation of the lipid sphingosine, creating S1P. S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. Two isoforms of SK have been identified, SK1 and SK2. The mRNA for both of these isoforms is widely expressed with SK1 expression highest in brain, heart, kidney, thymus, spleen and lung, while SK2 is highest in kidney and liver. SKs can be activated through growth factor, G protein-coupled, and immunoglobulin receptor signalling. SK1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. Regulation of SK1 may occur through ERK mediated phosphorylation of Ser-225. This phosphorylation leads to increased activity and translocation to the plasma membrane.

References

Pitson, S.M. et al. (2005) J Exp. Med. 201(1) :49.
Pitson, S.M. et al. (2003) EMBOJ. 22(20) :5491.
Melendez, A.J. et al. (2000) Gene 251(1) :19.

NCBI and Uniprot Product Information

• NCBI GI #: 289191342
• NCBI GeneID: 20698
• NCBI Accession #: NP_001165943.1
• NCBI GenBank Nucleotide #: NM_001172472.1
• UniProt Accession #: Q8CI15; O88886; Q3U2E3; Q91ZN3
• Molecular Weight: 42,344 Da
• NCBI Official Full Name: sphingosine kinase 1 isoform 2
• NCBI Official Synonym Full Names: sphingosine kinase 1
• NCBI Official Symbol: Sphk1
• NCBI Official Synonym Symbols: Sk1; Spk1; 1110006G24Rik
• NCBI Protein Information: sphingosine kinase 1
• UniProt Protein Name: Sphingosine kinase 1
• Protein Family: Sphingosine kinase
• UniProt Gene Name: Sphk1
• UniProt Synonym Gene Names: SK 1; SPK 1
• UniProt Entry Name: SPHK1_MOUSE

NCBI Description

This gene encodes a kinase that phosphorylates sphingosine into sphingosine-1-phosphate, which is involved in cell differentiation, motility, and apoptosis. The encoded protein plays a role in maintaining cellular levels of sphingosine-1-phosphate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]

Uniprot Description

SPHK1: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. Interacts with ACY1. Binds to calmodulin. Interacts with SPHKAP. Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - sphingolipid; Kinase, lipid; EC 2.7.1.91

Cellular Component: axon; cytoplasm; cytosol; membrane; neuron projection; nucleus; plasma membrane; synaptic vesicle

Molecular Function: ATP binding; calmodulin binding; D-erythro-sphingosine kinase activity; DNA binding; kinase activity; magnesium ion binding; nucleotide binding; protein phosphatase 2A binding; sphinganine kinase activity; transferase activity

Biological Process: activation of NF-kappaB transcription factor; blood vessel development; brain development; calcium-mediated signaling; cyclooxygenase pathway; inflammatory response; lipid phosphorylation; negative regulation of apoptosis; phosphorylation; positive regulation of angiogenesis; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of fibroblast proliferation; positive regulation of mitotic cell cycle; positive regulation of neurotransmitter secretion; positive regulation of NF-kappaB import into nucleus; positive regulation of protein amino acid phosphorylation; positive regulation of protein ubiquitination; positive regulation of smooth muscle contraction; regulation of interleukin-1 beta production; sphingosine biosynthetic process; sphingosine metabolic process