Typical Testing Data/Standard Curve (for reference only)
Mouse Sphingosine kinase 1 ELISA Kit | Sphk1 elisa kit
Mouse Sphingosine kinase 1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a kinase that phosphorylates sphingosine into sphingosine-1-phosphate, which is involved in cell differentiation, motility, and apoptosis. The encoded protein plays a role in maintaining cellular levels of sphingosine-1-phosphate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
Uniprot Description
SPHK1: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. Interacts with ACY1. Binds to calmodulin. Interacts with SPHKAP. Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Lipid Metabolism - sphingolipid; Kinase, lipid; EC 2.7.1.91
Cellular Component: axon; cytoplasm; cytosol; membrane; neuron projection; nucleus; plasma membrane; synaptic vesicle
Molecular Function: ATP binding; calmodulin binding; D-erythro-sphingosine kinase activity; DNA binding; kinase activity; magnesium ion binding; nucleotide binding; protein phosphatase 2A binding; sphinganine kinase activity; transferase activity
Biological Process: activation of NF-kappaB transcription factor; blood vessel development; brain development; calcium-mediated signaling; cyclooxygenase pathway; inflammatory response; lipid phosphorylation; negative regulation of apoptosis; phosphorylation; positive regulation of angiogenesis; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of fibroblast proliferation; positive regulation of mitotic cell cycle; positive regulation of neurotransmitter secretion; positive regulation of NF-kappaB import into nucleus; positive regulation of protein amino acid phosphorylation; positive regulation of protein ubiquitination; positive regulation of smooth muscle contraction; regulation of interleukin-1 beta production; sphingosine biosynthetic process; sphingosine metabolic process
Research Articles on Sphk1
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Product Notes
The Mouse Sphk1 sphk1 (Catalog #AAA288761) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA288761 ELISA Kit recognizes Mouse Sphk1. It is sometimes possible for the material contained within the vial of "Sphingosine kinase 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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