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Immunohistochemistry (IHC) (Immunohistochemical staining of rabbit brain using anti-SNAP25 antibody MBS488404 Formalin fixed rabbit brain slices were were stained with a MBS488404 at 3 ug/ml.)

SNAP-25 recombinant antibody

Anti-SNAP-25 [SP12]

Reactivity
Human, Mouse, Rat, Hamster, Pig, Cat, Guinea Pig, Cow
Applications
Immunohistochemistry, Western Blot, Immunofluorescence, ELISA
Purity
Protein A Affinity Purified
Synonyms
SNAP-25; Monoclonal Recombinant Antibody; Anti-SNAP-25 [SP12]; Synaptosomal nerve-associated protein 25; CMS18; RIC-4; RIC4; SEC9; SNAP; bA416N4.2; dJ1068F16.2; SUP; synaptosome associated protein 25kDal synaptosome associated protein 25; SNAP-25 recombinant antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse, Rat, Hamster, Pig, Cat, Guinea Pig, Cow
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Clone Number
SP12
Specificity
This antibody binds to native and denatured SNAP-25.
Purity/Purification
Protein A Affinity Purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Sequence Length
217
Applicable Applications for SNAP-25 recombinant antibody
Immunohistochemistry (IHC), Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Antibody Type
Recombinant Antibody
Antibody Species
Mouse
Original Species Name
Mouse
Buffer Composition
PBS only with 0.02% Proclin 300.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Immunohistochemistry (IHC)

(Immunohistochemical staining of rabbit brain using anti-SNAP25 antibody MBS488404 Formalin fixed rabbit brain slices were were stained with a MBS488404 at 3 ug/ml.)

Immunohistochemistry (IHC) (Immunohistochemical staining of rabbit brain using anti-SNAP25 antibody MBS488404 Formalin fixed rabbit brain slices were were stained with a MBS488404 at 3 ug/ml.)

Western Blot (WB)

(Western Blot using anti-SNAP25 antibody MBS488404 Mouse brain (lane 1), mouse rectum (lane 2), mouse pancreas (lane 3), human lung cancer (lane 4), human breast cancer (lane 5) and Hela cell(lane 6) samples were resolved on a 12% SDS PAGE gel and blots probed with MBS488404 at 2 ug/ml before being detected by a secondary antibody. Bands correspondig to the expected 23 kDa SNAP25 protein were detected in mouse brain (along with likely oligomers) as well as in mouse rectum (faint). In this experiment no SNAP25 was dected in the other samples.)

Western Blot (WB) (Western Blot using anti-SNAP25 antibody MBS488404 Mouse brain (lane 1), mouse rectum (lane 2), mouse pancreas (lane 3), human lung cancer (lane 4), human breast cancer (lane 5) and Hela cell(lane 6) samples were resolved on a 12% SDS PAGE gel and blots probed with MBS488404 at 2 ug/ml before being detected by a secondary antibody. Bands correspondig to the expected 23 kDa SNAP25 protein were detected in mouse brain (along with likely oligomers) as well as in mouse rectum (faint). In this experiment no SNAP25 was dected in the other samples.)
Related Product Information for SNAP-25 recombinant antibody
Recombinant monoclonal antibody to SNAP-25. Manufactured using the recombinant platform with variable regions (i.e. specificity) from the hybridoma SP12.
Product Categories/Family for SNAP-25 recombinant antibody
References
Wakabayashi et al. Synapse alterations in the hippocampal-entorhinal formation in Alzheimer's disease with and without Lewy body disease. Brain Res. 1994 Dec 19;667(1):24-32 PMID:7895080
Original Published Journal: Brain Res. 1994 Dec 19;667(1):24-32
Publication Note: Describes the use of this antibody in IHC on human brain affected by various pathologies (Alzheimer's Disease, Lewy body variant of Alzheimer's Disease and Lewy body disease).

NCBI and Uniprot Product Information

NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
SNAP-25

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Product Notes

The SNAP-25 (Catalog #AAA488404) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-SNAP-25 [SP12] reacts with Human, Mouse, Rat, Hamster, Pig, Cat, Guinea Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's SNAP-25 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB), Immunofluorescence (IF), ELISA (EIA). Researchers should empirically determine the suitability of the SNAP-25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SNAP-25, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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