Western Blot (WB)
(Western blot analysis of extracts from Hela cells(heat shock treatment), using MORC2 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit anti-Human MORC2 Polyclonal Antibody | anti-MORC2 antibody
MORC2 Antibody
Gene Names
MORC2; ZCW3; ZCWCC1
Reactivity
Human
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Synonyms
MORC2; Polyclonal Antibody; MORC2 Antibody; AC004542.C22.1.; CW type with coiled coil domain 1; KIAA0852; MORC family CW type zinc finger 2; MORC family CW-type zinc finger protein 2; MORC2_HUMAN; ZCW3; ZCWCC1; Zinc finger; Zinc finger CW type coiled coil domain protein 1; Zinc finger CW type with coiled coil domain 1; Zinc finger CW-type coiled-coil domain protein 1; Zing finger CW type 3; anti-MORC2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
MORC2 Antibody detects endogenous levels of total MORC2.
Tissue Specificity: Highly expressed in smooth muscle, pancreas and testis.
Tissue Specificity: Highly expressed in smooth muscle, pancreas and testis.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-MORC2 antibody
Western Blot (WB), Peptide ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Peptide ELISA: 1:20,000-1:40,000
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human MORC2.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Phosphorylated by PAK1 at Ser-739 upon DNA damage. Phosphorylation is required for ATPase activity and recruitment to damaged chromatin.
Subunit Structure
Homodimerizes upon ATP-binding and dissociate upon ATP hydrolysis; homodimerization is required for gene silencing. Interacts with HDAC4. Interacts with ACLY. Interacts with TASOR and MPHOSPH8; the interactions associate MORC2 with the HUSH complex which recruits MORC2 to heterochromatic loci.
Subcellular Location
Nucleus. Cytoplasm>Cytosol. Chromosome. Nucleus matrix.
Note: Mainly located in the nucleus (PubMed:20225202). Upon phosphorylation at Ser-739, recruited to damaged chromatin (PubMed:23260667).
Note: Mainly located in the nucleus (PubMed:20225202). Upon phosphorylation at Ser-739, recruited to damaged chromatin (PubMed:23260667).
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from Hela cells(heat shock treatment), using MORC2 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Hela cells(heat shock treatment), using MORC2 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-MORC2 antibody
Essential for epigenetic silencing by the HUSH (human silencing hub) complex. Recruited by HUSH to target site in heterochromatin, the ATPase activity and homodimerization are critical for HUSH-mediated silencing. Represses germ cell-related genes and L1 retrotransposons in collaboration with SETDB1 and the HUSH complex, the silencing is dependent of repressive epigenetic modifications, such as H3K9me3 mark. Silencing events often occur within introns of transcriptionally active genes, and lead to the down-regulation of host gene expression. During DNA damage response, regulates chromatin remodeling through ATP hydrolysis. Upon DNA damage, is phosphorylated by PAK1, both colocalize to chromatin and induce H2AX expression. ATPase activity is required and dependent of phosphorylation by PAK1 and presence of DNA. Recruits histone deacetylases, such as HDAC4, to promoter regions, causing local histone H3 deacetylation and transcriptional repression of genes such as CA9. Exhibits a cytosolic function in lipogenesis, adipogenic differentiation, and lipid homeostasis by increasing the activity of ACLY, possibly preventing its dephosphorylation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
117,823 Da
NCBI Official Full Name
MORC family CW-type zinc finger protein 2
NCBI Official Synonym Full Names
MORC family CW-type zinc finger 2
NCBI Official Symbol
MORC2
NCBI Official Synonym Symbols
ZCW3; ZCWCC1
NCBI Protein Information
MORC family CW-type zinc finger protein 2; zinc finger, CW type with coiled-coil domain 1; zinc finger, CW-type with coiled-coil domain 1; zinc finger CW-type coiled-coil domain protein 1
UniProt Protein Name
MORC family CW-type zinc finger protein 2
Protein Family
UniProt Gene Name
MORC2
UniProt Synonym Gene Names
KIAA0852; ZCWCC1
UniProt Entry Name
MORC2_HUMAN
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Product Notes
The MORC2 morc2 (Catalog #AAA9613523) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MORC2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MORC2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:500-1:2000 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the MORC2 morc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MORC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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