Western Blot (WB)
(Lane 2.: NFATc1 transfected 293T cell lysate )
Mouse anti-Human RBP4 Monoclonal Antibody | anti-RBP4 antibody
RBP4 antibody
Gene Names
RBP4; RDCCAS; MCOPCB10
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
Purity
By protein-G affinity chromatography
Synonyms
RBP4; Monoclonal Antibody; RBP4 antibody; Retinol binding protein 4; Retinol binding protein 4 Plasma retinol binding protein 4; prbp; PRO2222; Retinol binding protein 4 interstitial; Retinol binding protein 4 plasma; ; anti-RBP4 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT2B4
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
201
Applicable Applications for anti-RBP4 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:500 ~ 1000.
Recommended starting dilution is 1:500.
Recommended starting dilution is 1:500.
Antigen Species
Human
Immunogen
Recombinant human RBP4 (19-201aa) purified from E. coli
Clone Note
Anti-human RBP4 mAb, clone AT2B4, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human RBP4 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(Lane 2.: NFATc1 transfected 293T cell lysate )
Western Blot (WB)
(Lane 2.: NFATc1 transfected 293T cell lysate )
Immunofluorescence (IF)
(ICC/IF analysis of RBP4 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human RBP4 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF)
(ICC/IF analysis of RBP4 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human RBP4 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Related Product Information for anti-RBP4 antibody
Retinol binding protein 4 (RBP4) belongs to the lipocalin family and is the specific carrier for retinol in the blood. This protein was found to be expressed and secreted by adipose tissue, and was strongly associated with insulin resistance. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli.
Product Categories/Family for anti-RBP4 antibody
References
Tan Bk, et al., (2007) J Clin Endocrinol Metab 92 (7):2590-2597; Yao-Borengasser A, et al., (2007) J Clin Endocrinol Metab 92 (7):2430-2432
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,010 Da
NCBI Official Full Name
retinol-binding protein 4 isoform a
NCBI Official Synonym Full Names
retinol binding protein 4
NCBI Official Symbol
RBP4
NCBI Official Synonym Symbols
RDCCAS; MCOPCB10
NCBI Protein Information
retinol-binding protein 4
UniProt Protein Name
Retinol-binding protein 4
Protein Family
UniProt Gene Name
RBP4
UniProt Synonym Gene Names
PRBP; RBP
NCBI Description
This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. [provided by RefSeq, Jul 2008]
Uniprot Description
Retinol-binding protein that mediates retinol transport in blood plasma (PubMed:5541771). Delivers retinol from the liver stores to the peripheral tissues (Probable). Transfers the bound all-trans retinol to STRA6, that then facilitates retinol transport across the cell membrane (PubMed:22665496).
Research Articles on RBP4
Similar Products
Product Notes
The RBP4 rbp4 (Catalog #AAA200135) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RBP4 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RBP4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:500 ~ 1000. Recommended starting dilution is 1:500. Researchers should empirically determine the suitability of the RBP4 rbp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RBP4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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