Rabbit anti-Human PHLA2 Polyclonal Antibody | anti-PHLA2 antibody

PHLA2 Antibody

Cat. Num. AAA9600497

• Gene Names: PHLDA2; IPL; BRW1C; BWR1C; HLDA2; TSSC3
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: PHLA2; Polyclonal Antibody; PHLA2 Antibody; Beckwith Wiedemann syndrome chromosome region 1 candidate protein C; Beckwith-Wiedemann syndrome chromosomal region 1 candidate gene C protein; BRW 1C; BRW1C; BWR 1C; BWR1C; HLDA 2; HLDA2; Imprinted in placenta and liver; Imprinted in placenta and liver protein; IPL; p17 Beckwith Wiedemann region 1C; p17 BWR1C; p17-Beckwith-Wiedemann region 1 C; p17-BWR1C; PHLA2_HUMAN; PHLDA 2; phlda2; Pleckstrin homology like domain family A member 2; Pleckstrin homology-like domain family A member 2; TSSC 3; Tumor suppressing STF cDNA 3 protein; Tumor suppressing subchromosomal transferable fragment candidate gene 3 protein; Tumor suppressing subchromosomal transferable fragment cDNA 3; Tumor suppressing subtransferable candidate 3; Tumor supressing STF cDNA 3; Tumor-suppressing STF cDNA 3 protein; Tumor-suppressing subchromosomal transferable fragment candidate gene 3 protein; anti-PHLA2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PHLA2 antibody detects endogenous levels of PHLA2
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 152

• Applicable Applications for anti-PHLA2 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human PHLA2
• Subcellular Location: Cytoplasm. Membrane.
• Tissue Specificity: Expressed in placenta and adult prostate gland. In placenta, it is present in all cells of the villous cytotrophoblast. The protein is absent in cells from hydatidiform moles. Hydatidiform mole is a gestation characterized by abnormal development of both fetus and trophoblast. The majority of hydatidiform moles are associated with an excess of paternal to maternal genomes and are likely to result from the abnormal expression of imprinted genes (at protein level). Expressed at low levels in adult liver and lung, and fetal liver. Expressed in adult brain and neuroblastoma, medullablastoma and glioblastoma cell lines.
• Predicted Cross Reactivity: Pig, Bovine, Chicken
• Similarity: Pig (90%), Bovine (89%), Chicken (88%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HepG2, using PHLA2 Antibody. Lane 1 was treated with the antigen-specific peptide.)

Immunohistochemistry (IHC)

(MBS9600497 at 1/100 staining human Breast cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Related Product Information for anti-PHLA2 antibody

Description: This gene is one of several genes in the imprinted gene domain of 11p15.5 which is considered to be an important tumor suppressor gene region. Alterations in this region may be associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Studies of the mouse gene, however, which is also located in an imprinted gene domain, have shown that the product of this gene regulates placental growth.
Function: Plays a role in regulating placenta growth. May act via its PH domain that competes with other PH domain-containing proteins, thereby preventing their binding to membrane lipids (By similarity).
Similarity: The PH domain binds phosphoinositides with a broad specificity. It may compete with the PH domain of some other proteins, thereby interfering with their binding to phosphatidylinositol 4, 5-bisphosphate (PIP2) and phosphatidylinositol 3, 4, 5-trisphosphate (PIP3) (By similarity). Belongs to the PHLDA2 family.

NCBI and Uniprot Product Information

• NCBI GI #: 4507705
• NCBI GeneID: 7262
• NCBI Accession #: NP_003302.1
• NCBI GenBank Nucleotide #: NM_003311.3
• UniProt Accession #: Q53GA4; O00496
• Molecular Weight: Observed: 17 kDa; Predicted: 18 kDa
• NCBI Official Full Name: pleckstrin homology-like domain family A member 2
• NCBI Official Synonym Full Names: pleckstrin homology like domain family A member 2
• NCBI Official Symbol: PHLDA2
• NCBI Official Synonym Symbols: IPL; BRW1C; BWR1C; HLDA2; TSSC3
• NCBI Protein Information: pleckstrin homology-like domain family A member 2
• UniProt Protein Name: Pleckstrin homology-like domain family A member 2
• UniProt Gene Name: PHLDA2
• UniProt Synonym Gene Names: BWR1C; HLDA2; IPL; TSSC3; p17-BWR1C

NCBI Description

This gene is located in a cluster of imprinted genes on chromosome 11p15.5, which is considered to be an important tumor suppressor gene region. Alterations in this region may be associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. This gene has been shown to be imprinted, with preferential expression from the maternal allele in placenta and liver. [provided by RefSeq, Oct 2010]

Uniprot Description

Plays a role in regulating placenta growth. May act via its PH domain that competes with other PH domain-containing proteins, thereby preventing their binding to membrane lipids ().