Western Blot (WB)
(Anti-PPM1A rabbit monoclonal antibody at 1:500Lane A: Jurkat Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: SKBR3 Whole Cell LysateLane D: Mouse brain Tissue LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:42 kDaObserved band size:45 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Mouse PPM1A Monoclonal Antibody | anti-PPM1A antibody
Recombinant Anti-PPM1A Antibody, Rabbit Monoclonal
Gene Names
Ppm1a; MMPa-2; MPPa-1; AI427932; AU017636; 2310003C21Rik; 2900017D14Rik
Reactivity
Mouse
Applications
Western Blot
Purity
Protein A
Synonyms
PPM1A; Monoclonal Antibody; Recombinant Anti-PPM1A Antibody; Rabbit Monoclonal; PPM1A/PP2CA Antibody; Rabbit MAb; protein phosphatase; Mg2+/Mn2+ dependent; 1A; Anti-2310003C21Rik Antibody; Anti-2900017D14Rik Antibody; Anti-AI427932 Antibody; Anti-AU017636 Antibody; Anti-MMPa-2 Antibody; Anti-MPPa-1 Antibody; anti-PPM1A antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
006
Specificity
Mouse PPM1A/PP2CA
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-PPM1A antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Mouse PPM1A/PP2CA Protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse PPM1A/PP2CA (rM PPM1A/PP2CA).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-PPM1A rabbit monoclonal antibody at 1:500Lane A: Jurkat Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: SKBR3 Whole Cell LysateLane D: Mouse brain Tissue LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:42 kDaObserved band size:45 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-PPM1A rabbit monoclonal antibody at 1:500Lane A: Jurkat Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: SKBR3 Whole Cell LysateLane D: Mouse brain Tissue LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:42 kDaObserved band size:45 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-PPM1A antibody
Protein phosphatase 1A (PPM1A/PP2CA) is an enzyme belonging to the PP2C family of Ser/Thr protein phosphatases. Members of PP2C family are negative regulators of cell stress response pathways and the MAP kinases and MAP kinase kinases. It has also been demonstrated to inhibit the activation of p38 and JNK kinase cascades. PPM1A dephosphorylates and promotes nuclear export of TGFbeta-activated Smad2/3. Ectopic expression of PPM1A abolishes TGFbeta-induced antiproliferative and transcriptional responses, whereas depletion of PPM1A enhances TGFbeta signaling in mammalian cells. It has been demonstrated that PPM1A/PP2CA, through dephosphorylation of Smad2/3, plays a critical role in terminating TGFbeta signaling. Overexpression of PPM1A is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to cell apoptosis.
Product Categories/Family for anti-PPM1A antibody
References
Lin X, et al. (2006) PPM1A functions as a Smad phosphatase to terminate TGFbeta signaling. Cell. 125(5): 915-28.Marc F, et al. (2003) Protein phosphatase 2C binds selectively to and dephosphorylates metabotropic glutamate receptor 3. Proc Natl Acad. 100 (26): 16006-11.Mann DJ, et al. (1992) Mammalian protein serine/threonine phosphatase 2C: cDNA cloning and comparative analysis of amino acid sequences. Biochim Biophys Acta. 1130 (1): 100-4.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42,433 Da
NCBI Official Full Name
protein phosphatase 1A
NCBI Official Synonym Full Names
protein phosphatase 1A, magnesium dependent, alpha isoform
NCBI Official Symbol
Ppm1a
NCBI Official Synonym Symbols
MMPa-2; MPPa-1; AI427932; AU017636; 2310003C21Rik; 2900017D14Rik
NCBI Protein Information
protein phosphatase 1A; Mpp alpha; PP2C-alpha; protein phosphatase IA; protein phosphatase 2C isoform alpha
UniProt Protein Name
Protein phosphatase 1A
Protein Family
UniProt Gene Name
Ppm1a
UniProt Synonym Gene Names
Pppm1a; PP2C-alpha
UniProt Entry Name
PPM1A_MOUSE
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Product Notes
The PPM1A ppm1a (Catalog #AAA8111306) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-PPM1A Antibody, Rabbit Monoclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PPM1A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the PPM1A ppm1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPM1A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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