PPM1A recombinant protein

PPM1A Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122658

• Gene Names: Ppm1a; MMPa-2; MPPa-1; AI427932; AU017636; 2310003C21Rik; 2900017D14Rik
• Purity: >98% as determined by SDS-PAGE
• Synonyms: PPM1A; PPM1A Protein; Mouse; Recombinant (His Tag); Mouse PPM1A/PP2CA/PP2C-alpha Protein (His Tag); 2310003C21Rik Protein; 2900017D14Rik Protein; AI427932 Protein; AU017636 Protein; MMPa-2 Protein; MPPa-1 Protein; protein phosphatase; Mg2+/Mn2+ dependent; 1A; PPM1A recombinant protein

• Host: E Coli
• Purity/Purification: >98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 10mM HEPES, 500 NaCl, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Trp382

• Species: Mouse
• Predicted N Terminal: Met 1
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse PPM1A (NP_032936.1)(Met 1-Trp 382) was expressed, with a polyhistide tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PPM1A recombinant protein

Background: Protein phosphatase 1A (PPM1A/PP2CA) is an enzyme belonging to the PP2C family of Ser/Thr protein phosphatases. Members of PP2C family are negative regulators of cell stress response pathways and the MAP kinases and MAP kinase kinases. It has also been demonstrated to inhibit the activation of p38 and JNK kinase cascades. PPM1A dephosphorylates and promotes nuclear export of TGFbeta-activated Smad2/3. Ectopic expression of PPM1A abolishes TGFbeta-induced antiproliferative and transcriptional responses, whereas depletion of PPM1A enhances TGFbeta signaling in mammalian cells. It has been demonstrated that PPM1A/PP2CA, through dephosphorylation of Smad2/3, plays a critical role in terminating TGFbeta signaling. Overexpression of PPM1A is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to cell apoptosis.

Product Categories/Family for PPM1A recombinant protein

Phosphatases and Regulators

References

Lin X, et al. (2006) PPM1A functions as a Smad phosphatase to terminate TGFbeta signaling. Cell. 125(5): 915-28.Marc F, et al. (2003) Protein phosphatase 2C binds selectively to and dephosphorylates metabotropic glutamate receptor 3. Proc Natl Acad. 100 (26): 16006-11.Mann DJ, et al. (1992) Mammalian protein serine/threonine phosphatase 2C: cDNA cloning and comparative analysis of amino acid sequences. Biochim Biophys Acta. 1130 (1): 100-4.

NCBI and Uniprot Product Information

• NCBI GI #: 6679443
• NCBI GeneID: 19042
• NCBI Accession #: NP_032936.1
• NCBI GenBank Nucleotide #: NM_008910.3
• UniProt Accession #: P49443
• Molecular Weight: 42,433 Da
• NCBI Official Full Name: protein phosphatase 1A
• NCBI Official Synonym Full Names: protein phosphatase 1A, magnesium dependent, alpha isoform
• NCBI Official Symbol: Ppm1a
• NCBI Official Synonym Symbols: MMPa-2; MPPa-1; AI427932; AU017636; 2310003C21Rik; 2900017D14Rik
• NCBI Protein Information: protein phosphatase 1A; Mpp alpha; PP2C-alpha; protein phosphatase IA; protein phosphatase 2C isoform alpha
• UniProt Protein Name: Protein phosphatase 1A
• Protein Family: Protein phosphatase
• UniProt Gene Name: Ppm1a
• UniProt Synonym Gene Names: Pppm1a; PP2C-alpha
• UniProt Entry Name: PPM1A_MOUSE