PPM1A / PP2CA / PP2C-alpha Recombinant Protein | PP2CA recombinant protein

Recombinant Mouse PPM1A / PP2CA / PP2C-alpha Protein (His tag)

Cat. Num. AAA2547060

• Gene Names: Ppm1a; MMPa-2; MPPa-1; AI427932; AU017636; 2310003C21Rik; 2900017D14Rik
• Purity: > 98 % as determined by SDS-PAGE
• Synonyms: PPM1A / PP2CA / PP2C-alpha; Recombinant Mouse PPM1A / PP2CA / PP2C-alpha Protein (His tag); Ppm1; 2310003C21Rik; 2900017D14Rik; AI427932; AU017636; MMPa-2; MPPa-1; PP2CA recombinant protein

• Host: E Coli
• Purity/Purification: > 98 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 10mM HEPES, 500 NaCl, pH 7.5
• Sequence Length: 382

• Application Notes: The recombinant mouse PPM1A consisting of 388 amino acids and has a calculated molecular mass of 43.3 kDa as estimated in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met 1
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for PP2CA recombinant protein

Background: Protein phosphatase 1A (PPM1A / PP2CA) is an enzyme belonging to the PP2C family of Ser / Thr protein phosphatases. Members of PP2C family are negative regulators of cell stress response pathways and the MAP kinases and MAP kinase kinases. It has also been demonstrated to inhibit the activation of p38 and JNK kinase cascades. PPM1A dephosphorylates and promotes nuclear export of TGFbeta-activated Smad2/3. Ectopic expression of PPM1A abolishes TGFbeta-induced antiproliferative and transcriptional responses, whereas depletion of PPM1A enhances TGFbeta signaling in mammalian cells. It has been demonstrated that PPM1A / PP2CA, through dephosphorylation of Smad2/3, plays a critical role in terminating TGFbeta signaling. Overexpression of PPM1A is reported to activate the expression of the tumor suppressor gene TP53 / p53, which leads to cell apoptosis.

Description: A DNA sequence encoding the mouse PPM1A (NP_032936.1) (Met 1-Trp 382) was expressed, with a polyhistide tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 6679443
• NCBI GeneID: 19042
• NCBI Accession #: NP_032936.1
• NCBI GenBank Nucleotide #: NM_008910.3
• UniProt Accession #: P49443
• Molecular Weight: 42,433 Da
• NCBI Official Full Name: protein phosphatase 1A
• NCBI Official Synonym Full Names: protein phosphatase 1A, magnesium dependent, alpha isoform
• NCBI Official Symbol: Ppm1a
• NCBI Official Synonym Symbols: MMPa-2; MPPa-1; AI427932; AU017636; 2310003C21Rik; 2900017D14Rik
• NCBI Protein Information: protein phosphatase 1A
• UniProt Protein Name: Protein phosphatase 1A
• Protein Family: Protein phosphatase
• UniProt Gene Name: Ppm1a
• UniProt Synonym Gene Names: Pppm1a; PP2C-alpha

Uniprot Description

PPPM1A: a Ser/Thr protein phosphatase of the PP2C family. Inhibits the activation of p38 and JNK kinase cascades induced by environmental stresses. Can also dephosphorylate cyclin-dependent kinases and may play a role in cell cycle control. Overexpression of this phosphatase activates the expression of p53, leading to G2/M cell cycle arrest and apoptosis.

Protein type: EC 3.1.3.16; Motility/polarity/chemotaxis; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 12|12 C3

Cellular Component: cytosol; membrane; neuron projection; nucleus; voltage-gated calcium channel complex

Molecular Function: calmodulin-dependent protein phosphatase activity; phosphoprotein phosphatase activity; protein C-terminus binding; protein serine/threonine phosphatase activity; signal transducer activity

Biological Process: dephosphorylation; N-terminal protein myristoylation; negative regulation of BMP signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of NF-kappaB import into nucleus; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of protein export from nucleus; positive regulation of transcription, DNA-dependent; positive regulation of Wnt receptor signaling pathway; protein amino acid dephosphorylation; Wnt receptor signaling pathway