Testing Data
Mouse PKC q Monoclonal Antibody
PKC-theta (N-terminal region)
Reactivity
Human, Rat, Mouse
Applications
ELISA, Western Blot
Purity
Protein A Purified
Synonyms
PKC q; Monoclonal Antibody; PKC-theta (N-terminal region); PKC q (N-terminal Region); PKC theta; anti-PKC q antibody
Host
Mouse
Reactivity
Human, Rat, Mouse
Clonality
Monoclonal
Isotype
IgG2a
Specificity
This antibody detects a 79 kDa* protein corresponding to the molecular mass of PKCq on SDS-PAGE immunoblots of human Jurkat cell lysates.
Purity/Purification
Protein A Purified
Form/Format
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ml phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-PKC q antibody
ELISA, Western Blot
Application Notes
ELISA: 1:2000
Western Blot: 1:1000
Western Blot: 1:1000
Immunogen
Clone (M217) was generated from a recombinant mouse PKCq protein that included amino acids residues in the N-terminal region. This sequence is conserved in human and rat PKCq, and has low homology to other PKC family members.
Preparation and Storage
Store at -20 degree C. Do not aliquot. Stable for 1 year.
Testing Data
Testing Data
Related Product Information for anti-PKC q antibody
The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalytic domain. The conventional PKC subfamily (a, bI, bII, and g) is regulated by both Ca2+ and diacylglycerol. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters, and growth factors. The phosphorylation of multiple sites in PKCs regulates their activity.
References
• Kawakami et al. (2003) Proc. Natl. Acad. Sci. USA 100:9470-9475.
• Nishizuka, Y. (1988) Nature 334:661.
• Nishizuka, Y. (1988) Nature 334:661.
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Product Notes
The PKC q (Catalog #AAA470025) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PKC-theta (N-terminal region) reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PKC q can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. ELISA: 1:2000 Western Blot: 1:1000. Researchers should empirically determine the suitability of the PKC q for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKC q, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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