Mouse Endothelial Nitric Oxide Synthase (C-terminal Region) Monoclonal Antibody | anti-NOS3 antibody

Endothelial Nitric Oxide Synthase (C-terminal Region)

Cat. Num. AAA470011

• Gene Names: NOS3; eNOS; ECNOS
• Reactivity: Human, Rat, Mouse
• Applications: ELISA, Western Blot
• Purity: Protein A Purified
• Synonyms: Endothelial Nitric Oxide Synthase (C-terminal Region); Monoclonal Antibody; endothelial Nitric Oxide Synthase; eNOS; ecNOS; NOS-III; anti-NOS3 antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: The antibody detects a 140 kDa* protein corresponding to eNOS on SDS-PAGE immunoblots of human umbilical vein endothelial cells.
• Purity/Purification: Protein A Purified
• Form/Format: Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ml phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-NOS3 antibody: ELISA, Western Blot
• Application Notes: ELISA: 1:2000; Western Blot: 1:1000
• Immunogen: Clone (M221) was generated from a recombinant mouse eNOS protein that included amino acids residues in the C-terminal region. This sequence is conserved in human and rat eNOS, and has low homology to other NOS family members.
• Preparation and Storage: Store at -20 degree C. Do not aliquot. Stable for 1 year.

Testing Data

Related Product Information for anti-NOS3 antibody

Nitric oxide (NO) has a broad range of biological activities and is implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOS), the enzymes responsible for synthesis of NO, are homodimers whose monomers are themselves two fused enzymes: a cytochrome reductase and a cytochrome that requires three cosubstrates (L-arginine, NADPH, and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme). Several distinct NOS isoforms are produced from three distinct genes. The inducible form of NOS, iNOS (NOS-II), is Ca2+ independent and is expressed in a broad range of cell types, and two constitutive Ca2+/CaM-dependent forms of NOS: nNOS (bNOS, NOS-I) identified in neurons and eNOS (ecNOS, NOS-III) identified in endothelial cells. Regulation of eNOS activity occurs through phosphorylation at multiple sites. Phosphorylation of Ser-633 (mouse Ser-632) in the FMN binding domain increases eNOS activity and may be important for the maintenance of NO synthesis after initial activation by Ca(2+) flux and Ser-1177 phosphorylation.

References

• Xie, Q.W. et al. (1992) Science 256:225.
• Musicki, B. et al. (2005) Proc. Natl. Acad. Sci.102(33):11870.
• Mount, P.F. et al. (2007) J Mo.l Cell. Cardiol. 42(2):271.

NCBI and Uniprot Product Information

• NCBI GI #: 825652
• NCBI GeneID: 4846
• UniProt Accession #: P29474; Q13662; Q14251; Q14434; Q548C1; Q6GSL5; Q9UDC6; A8KA63; B2RCQ1; O14937; Q96J98; Q96P37
• Molecular Weight: 133,289 Da
• NCBI Official Full Name: endothelial nitric oxide synthase
• NCBI Official Synonym Full Names: nitric oxide synthase 3 (endothelial cell)
• NCBI Official Symbol: NOS3
• NCBI Official Synonym Symbols: eNOS; ECNOS
• NCBI Protein Information: nitric oxide synthase, endothelial; cNOS; EC-NOS; NOSIII; NOS type III; endothelial NOS; constitutive NOS; OTTHUMP00000212826; OTTHUMP00000213183; OTTHUMP00000213184; OTTHUMP00000213185
• UniProt Protein Name: Nitric oxide synthase, endothelial
• Protein Family: Nitric oxide synthase
• UniProt Gene Name: NOS3
• UniProt Entry Name: NOS3_HUMAN

NCBI Description

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

Function: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

Catalytic activity: L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+.

Cofactor: Heme group.Binds 1 FAD.Binds 1 FMN.Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.

Enzyme regulation: Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN. Ref.19 Ref.20

Subunit structure: Homodimer. Interacts with NOSIP and NOSTRIN. Ref.19 Ref.20

Subcellular location: Cell membrane. Membrane › caveola. Cytoplasm › cytoskeleton. Golgi apparatus. Note: Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity. Ref.19 Ref.20 Ref.21 Ref.22

Tissue specificity: Platelets, placenta, liver and kidney. Ref.18

Polymorphism: Variation in NOS3 seem to be associated with susceptibility to coronary spasm.

Sequence similarities: Belongs to the NOS family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain.