Flow Cytometry
(Flow Cytometric analysis using TdT antibodyStaining of C57BL/6 thymocytes with CD4 antibody (FITC) and CD8a antibody PErCP-Cy5.5 followed by fixation permeabilization with the Foxp3 Stainng Buffers and subsequent staining with 0.25 ug of Anti-Mouse TdT (PE) (purple histogram). CD4 single positives are shown in gray and CD8 single positives in green.)
Mouse TdT Monoclonal Antibody | anti-TdT antibody
TdT antibody (PE)
Applications
Flow Cytometry
Synonyms
TdT; Monoclonal Antibody; TdT antibody (PE); Monoclonal TdT; Anti-TdT; terminal deoxynucleotidyl transferase; Anti TdT; anti-TdT antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b kappa
Clone Number
19-3
Specificity
Mouse
Form/Format
Liquid in PBS, pH 7.2, with 0.1% Gelatin and 0.09% Sodium Azide
Concentration
0.2 mg/mL (varies by lot)
Applicable Applications for anti-TdT antibody
Flow Cytometry (FC)
Application Notes
FC: 5 ul (0.25ug)
This can be used at the same concentration as the experimental antibody
A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100ul. Cell number should be determined empirically but can range from 105 to 108 cells/test.
This can be used at the same concentration as the experimental antibody
A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100ul. Cell number should be determined empirically but can range from 105 to 108 cells/test.
Biological Significance
This 19-3 monoclonal antibody recognizes human and mouse terminal-deoxynucleotidyl transferase, a 60 kDa polymerase responsible for the template-independent addition of N-nucleotides at gene segment junctions of developing lymphocytes. Expression is found in the nucleus of immature lymphocytes but not in mature lymphocytes or non-lymphoid cells. Tdt plays a critical role during TCR and Ig gene rearrangement. Regulation of expression in the mouse thymus correlates with T cell selection decreased or absent after positive selection. Additionally Tdt has been shown to be present in lymphoma or lymphoblastic leukemia cells.
Tag/Conjugate
PE
Species Reactivity/Cross-Reactivity
Human, Mouse
Preparation and Storage
4°C, store in dark, DO NOT FREEZE
Flow Cytometry
(Flow Cytometric analysis using TdT antibodyStaining of C57BL/6 thymocytes with CD4 antibody (FITC) and CD8a antibody PErCP-Cy5.5 followed by fixation permeabilization with the Foxp3 Stainng Buffers and subsequent staining with 0.25 ug of Anti-Mouse TdT (PE) (purple histogram). CD4 single positives are shown in gray and CD8 single positives in green.)
Flow Cytometry
(Flow Cytometric analysis using TdT antibodyStaining of C57BL/6 thymocytes with CD4 antibody (FITC) and CD8a antibody PErCP-Cy5.5 followed by fixation permeabilization with the Foxp3 Stainng Buffers and subsequent staining with 0.25 ug of Anti-Mouse TdT (PE) (purple histogram). CD4 single positives are shown in gray and CD8 single positives in green.)
Related Product Information for anti-TdT antibody
Mouse monoclonal TdT antibody (PE)
Product Categories/Family for anti-TdT antibody
Similar Products
Product Notes
The TdT (Catalog #AAA834070) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TdT can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC). FC: 5 ul (0.25ug) This can be used at the same concentration as the experimental antibody A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. Researchers should empirically determine the suitability of the TdT for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TdT, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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