Testing Data
Mouse Akt (N-terminal Region) Monoclonal Antibody
Akt (N-terminal Region)
Reactivity
Human, Rat, Mouse
Applications
ELISA, Western Blot, Immunocytochemistry
Purity
Protein A Purified
Synonyms
Akt (N-terminal Region); Monoclonal Antibody; PKBalpha; Protein Kinase B; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; anti-Akt (N-terminal Region) antibody
Host
Mouse
Reactivity
Human, Rat, Mouse
Clonality
Monoclonal
Isotype
IgG1
Specificity
This antibody detects a 60 kDa* protein corresponding to the apparent molecular mass of Akt on SDS-PAGE immunoblots of A431 cell lysate. Similar results were seen in various other human, rat, and mouse lysates.
Purity/Purification
Protein A Purified
Form/Format
Mouse monoclonal purified with protein A chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-Akt (N-terminal Region) antibody
ELISA, Western Blot, Immunocytochemistry
Application Notes
ELISA: 1:2000
Immunocytochemistry: 1:250
Western Blot: 1:1000
Immunocytochemistry: 1:250
Western Blot: 1:1000
Immunogen
Clone M101 was generated from a recombinant protein containing amino acid residues in the N-terminal region of human Akt1. This sequence is
highly conserved in human and mouse Akt, and may recognize Akt2 and Akt3.
Preparation and Storage
Store at -20 degree C. Do not aliquot. Stable for 1 year.
Testing Data
Testing Data
Related Product Information for anti-Akt (N-terminal Region) antibody
Akt (PKB, Rac kinase) is a 60kDa ser/thr kinase critical for controlling diverse cellular functions, including glucose metabolism, gene transcription, cell proliferation, and apoptosis. Akt phosphorylates a number of substrates including MBP, glycogen synthetase, PKA RII subunit, and histone H1. Akt is activated in response to insulin and growth factors in a PI3-kinase dependent manner. Activation of PI3-Kinase generates phosphatidylinositol 3,4-bisphosphate, which induces membrane translocation of Akt coincident with its phosphorylation at Thr-308 and Ser-473. Upon activation, Akt associates with members of the PKC family of kinases, such as PKC-delta and PKC-zeta. Ceramide-activated PKC-zeta leads to phosphorylation of Thr-34 within the pleckstrin homology domain of Akt. This phosphorylation inhibits PIP3 binding to Akt preventing activation of the kinase and may lead to cermide-induced cell death.
References
• Jones, P.F. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4171-4175.
• Marte, B. & Downward. J. (1997) TIBS. 22:355-358.
• Powell, D.J. et al. (2003) Mol. Cell Biol. 23:7794-7808.
• Smith, M.A. et al. (2007) Am. J. Phys. Cell. Phys. 293:1947. (WB: mouse C2C12)
• Moylan, J.S. et al. (2008) Am. J. Phys. Cell. Phys. 295:986. (WB: mouse C2C12)
• Marte, B. & Downward. J. (1997) TIBS. 22:355-358.
• Powell, D.J. et al. (2003) Mol. Cell Biol. 23:7794-7808.
• Smith, M.A. et al. (2007) Am. J. Phys. Cell. Phys. 293:1947. (WB: mouse C2C12)
• Moylan, J.S. et al. (2008) Am. J. Phys. Cell. Phys. 295:986. (WB: mouse C2C12)
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Product Notes
The Akt (N-terminal Region) (Catalog #AAA470001) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Akt (N-terminal Region) reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Akt (N-terminal Region) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunocytochemistry. ELISA: 1:2000 Immunocytochemistry: 1:250 Western Blot: 1:1000. Researchers should empirically determine the suitability of the Akt (N-terminal Region) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Akt (N-terminal Region), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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