Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant PDE4B protein using PDE4B antibody)
Mouse PDE4B Monoclonal Antibody | anti-PDE4B antibody
PDE4B antibody
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
PDE4B antibody was purified by affinity chromatography.
Synonyms
PDE4B; Monoclonal Antibody; PDE4B antibody; Monoclonal PDE4B; Anti-PDE4B; DKFZp686F2182; DPDE4; MGC126529; PDE4B5; PDEIVB; PDEB 4; PDEB-4; anti-PDE4B antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1D12
Purity/Purification
PDE4B antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
0.5-1 mg/ml (varies by lot)
Applicable Applications for anti-PDE4B antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IF: 1:100
IHC: 1:150
WB: 1:500-2000
IHC: 1:150
WB: 1:500-2000
Biological Significance
This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. Cyclic nucleotides are important second messengers that regulate and mediate a number of cellular responses to extracellular signals, such as hormones, light, and neurotransmitters. The cyclic nucleotide phosphodiesterases (PDEs) regulate the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. This gene encodes a protein that specifically hydrolyzes cAMP. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder.
Cross-Reactivity
Human, Mouse
Immunogen
PDE4B antibody was raised in Mouse using full length human recombinant protein of human PDE4B produced in HEK293T cell as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant PDE4B protein using PDE4B antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant PDE4B protein using PDE4B antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PDE4B protein (Right) or empty vector (Left) detected with PDE4B antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PDE4B protein (Right) or empty vector (Left) detected with PDE4B antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PDE4B protein in paraffin embedded Adenocarcinoma of Human ovary tissue using PDE4B antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PDE4B protein in paraffin embedded Adenocarcinoma of Human ovary tissue using PDE4B antibody)
Related Product Information for anti-PDE4B antibody
Mouse monoclonal PDE4B antibody
Product Categories/Family for anti-PDE4B antibody
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Product Notes
The PDE4B (Catalog #AAA832345) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PDE4B can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). IF: 1:100 IHC: 1:150 WB: 1:500-2000. Researchers should empirically determine the suitability of the PDE4B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDE4B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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