Mouse anti-Human TAF9L Monoclonal Antibody | anti-TAF9L antibody

Mouse monoclonal antibody Anti-Human TAF9L

Cat. Num. AAA120263

• Gene Names: TAF9B; DN7; DN-7; TAF9L; TAFII31L; TFIID-31
• Reactivity: Human
• Applications: Dot Blot, Immunocytochemistry, Western Blot
• Synonyms: TAF9L; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human TAF9L; Homo sapiens TAF9B RNA polymerase II; TATA box binding protein?(TBP)-associated factor; 31kDa (TAF9B); TAF9B;DN7; DN-7;TAFII31L; TFIID-31; anti-TAF9L antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 3365C4a
• Concentration: 100 ug/ml (1.0 ml) (varies by lot)
• Sequence Length: 2714

• Applicable Applications for anti-TAF9L antibody: Dot Blot (DB), Immunocytochemistry (ICC), Western Blot (WB)
• Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
• Sterility: Filtered through a 0.22 um membrane.

Quality Control

(Western blot analysis of immunized recombinant protein, using anti-TAF9L monoclonal antibody. )

Quality Control #2

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Western Blot (WB)

(Detection of TAF9L by Western blot.Samples: Whole cell lysate from human HEK293 (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. 3365C4a-1]Predicted molecular weight: 27 kDa)

Immunocytochemistry (ICC)

(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-TAF9L mAb. [Lot No. 3365C4a-1])

Related Product Information for anti-TAF9L antibody

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a protein that is similar to one of the small subunits of TFIID, TBP-associated factor 9, and is also a subunit of TFIID. TAF9 and TAF9b share some functions but also have distinct roles in the transcriptional regulatory process. [NCBI Entrez Gene Summary]

NCBI and Uniprot Product Information

• NCBI GI #: 85062626
• NCBI GeneID: 51616
• NCBI Accession #: NP_057059.2
• NCBI GenBank Nucleotide #: NM_015975
• Molecular Weight: 27,622 Da
• NCBI Official Full Name: Homo sapiens TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa (TAF9B), mRNA
• NCBI Official Synonym Full Names: TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa
• NCBI Official Symbol: TAF9B
• NCBI Official Synonym Symbols: DN7; DN-7; TAF9L; TAFII31L; TFIID-31
• NCBI Protein Information: transcription initiation factor TFIID subunit 9B; TAF9-like RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa; TBP-associated factor 9L; neuronal cell death-related protein 7; transcription associated factor TAFII31L; transcription initiation factor IID, 31kD subunit; transcription-associated factor TAFII31L
• UniProt Protein Name: Transcription initiation factor TFIID subunit 9B
• UniProt Gene Name: TAF9B
• UniProt Entry Name: TAF9B_HUMAN

NCBI Description

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a protein that is similar to one of the small subunits of TFIID, TBP-associated factor 9, and is also a subunit of TFIID. TAF9 and TAF9b share some functions but also have distinct roles in the transcriptional regulatory process. [provided by RefSeq, Jul 2008]

Uniprot Description

TAF9B: Essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. May have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription. Belongs to the TAF9 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: Xq13.1-q21.1

Cellular Component: nucleoplasm; transcription factor TFIID complex

Molecular Function: protein binding; protein heterodimerization activity; transcription corepressor activity

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; protein stabilization; viral reproduction; RNA elongation from RNA polymerase II promoter; gene expression; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell growth; negative regulation of apoptosis