Immunofluorescence (IF)
(Immunofluorescence staining of Human SPARCL1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human SPARCL1 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to cytoplasm.)
Mouse anti-Human SPARCL1 Monoclonal Antibody | anti-SPARCL1 antibody
Anti-SPARCL1 Antibody, Mouse Monoclonal
Gene Names
SPARCL1; SC1; MAST9; PIG33; MAST 9
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
Purity
Protein A
Synonyms
SPARCL1; Monoclonal Antibody; Anti-SPARCL1 Antibody; Mouse Monoclonal; SPARCL1/MAST9 Antibody; Mouse MAb; SPARC like 1; Anti-MAST 9 Antibody; Anti-MAST9 Antibody; Anti-PIG33 Antibody; Anti-SC1 Antibody; anti-SPARCL1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
6D1D6G5
Specificity
Human SPARCL1/MAST9
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-SPARCL1 antibody
ELISA (EIA), Flow Cytometry (FC/FACS/FCM), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
ELISA: 1:1000-1:2000
ICC/IF: 1:20-1:100
FC/FACS/FCM: 1:25-1:100
ICC/IF: 1:20-1:100
FC/FACS/FCM: 1:25-1:100
Immunogen
Recombinant Human SPARCL1 protein
Conjugation
Unconjugated
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SPARCL1 (rhSPARCL1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of Human SPARCL1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human SPARCL1 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to cytoplasm.)
Immunofluorescence (IF)
(Immunofluorescence staining of Human SPARCL1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human SPARCL1 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to cytoplasm.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human SPARCL1 expression on HL60 cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human SPARCL1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human SPARCL1 expression on HL60 cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human SPARCL1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Related Product Information for anti-SPARCL1 antibody
SPARC-like protein 1 (SPARCL1; also known as SC1, high endothelial venule protein, or hevin) is an extracellular matrix-associated, secreted glycoprotein belonging to the secreted protein acidic and rich in cysteine (SPARC) family of matricellular proteins. It contains three conserved structural domains that are implicated in the regulation of cell adhesion, migration, and proliferation. SPARCL1 is expressed during embryogenesis and tissue remodeling and is especially prominent in brain and vasculature. Its down-regulation in a number of cancers and the possibility of its functional compensation by SPARC has led to recent interest in hevin as a tumor suppressor and regulator of angiogenesis. SPARCL1 has antiadhesive properties, and loss of SPARCL1 expression is associated with increased proliferative activity and cell cycle progression. It is suggested that it may influence multiple cellular processes during distinct stages of brain development and function. Besides, SPARCL1 can influence the function of astroglial cells in the developing and mature central nervous system (CNS).
References
Sullivan MM, et al. (2004) Hevin/SC1, a matricellular glycoprotein and potential tumor-suppressor of the SPARC/BM-40/Osteonectin family. Int J Biochem Cell Biol. 36(6): 991-6.Esposito I, et al. (2007) Tumor-suppressor function of SPARC-like protein 1/Hevin in pancreatic cancer. Neoplasia. 9(1): 8-17.Weimer JM, et al. (2008) A BAC transgenic mouse model to analyze the function of astroglial SPARCL1 (SC1) in the central nervous system. Glia. 56(9): 935-41.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
75,208 Da
NCBI Official Full Name
SPARC-like protein 1 isoform 1
NCBI Official Synonym Full Names
SPARC-like 1 (hevin)
NCBI Official Symbol
SPARCL1
NCBI Official Synonym Symbols
SC1; MAST9; PIG33; MAST 9
NCBI Protein Information
SPARC-like protein 1; high endothelial venule protein; proliferation-inducing protein 33
Protein Family
Research Articles on SPARCL1
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Product Notes
The SPARCL1 (Catalog #AAA8100402) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-SPARCL1 Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SPARCL1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS/FCM), Immunocytochemistry (ICC), Immunofluorescence (IF). ELISA: 1:1000-1:2000 ICC/IF: 1:20-1:100 FC/FACS/FCM: 1:25-1:100. Researchers should empirically determine the suitability of the SPARCL1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPARCL1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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