Mouse anti-Human PD-1 Monoclonal Antibody | anti-PD-1 antibody

PD-1

Cat. Num. AAA344001

• Gene Names: PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
• Reactivity: Human
• Synonyms: PD-1; Monoclonal Antibody; anti-PD-1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Form/Format: Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide
• Sequence Length: 288

• Application Notes: The recommended working dilution range is 1:100 - 1:200.
• Positive Control: Tonsil, Lymph Node
• Principles and Procedures: Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. Results are interpreted using a light microscope.
• Reconstitution: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.; The concentrated antibody requires dilution in the optimized buffer, to the recommended working dilution range.
• Specimen Collection and Preparation for Analysis: Each tissue section should be fixed with 10% neutral buffered formalin, cut to the applicable thickness (4um), and placed on a glass slide that is positively charged. The prepared slide may then be baked for a minimum of 30 minutes in a 53-65 degree C oven (do not exceed 24 hours).; Note: Performance evaluation has been shown on human tissues only. Variable results may occur due to extended fixation time or special processes of specific tissue preparations.
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.; To ensure proper stability and delivery of the antibody after each run, replace the cap and immediately place the bottle in a refrigerator in an upright position.; Positive and negative controls should be simultaneously run with unknown specimens.

Immunohistochemistry (IHC)

(PD-1 [IHC001] on Tonsil)

Related Product Information for anti-PD-1 antibody

Intended Use: The PD-1 [IHC001] antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test methods.
Description: Programmed Death 1 (PD-1) is a member of the CD28/CTLA-4 family of T-cell regulators, expressed as a co-receptor on the surface of activated T-cells, B-cells, and macrophages. New studies have suggested that the PD-1/PD-L1 signaling pathway may be linked to anti-tumor immunity, as PD-L1 has been shown to induce apoptosis of activated T cells or inhibit activity of cytotoxic T cells. In comparison to CD10 and Bcl-6, PD-1 is expressed by fewer B cells and has therefore been considered a more specific and useful marker for angioimmunoblastic T-cell lymphoma. Therapies targeted toward the PD-1 receptor have shown remarkable clinical responses in patients with various types of cancer, including non-small-cell lung cancer, melanoma, and renal-cell cancer.

References

1. Dorfman DM, et al. Am J Surg Pathol. 2006; 30:802-10.
2. Hamanishi J, et al. Proc Natl Acad Sci USA. 2007; 104:3360-5.
3. Kobayashi M, et al. J Rheumatol. 2005; 32:2156-63.
4. Konishi J, et al. Clin Cancer Res. 2004; 10:5094-100.
5. Mataki N, et al. Am J Gastroenterol. 2007; 102:302-12.
6. Kim JW, et al. Oncology (Williston Park). 2014; 28:15-28.
7. Tumeh PC, et al. Nature. 2014; 515:568-71.
8. D'Incecco A, et al. Br J Cancer. 2015; 112:95-102.
9. Tykodi SS. Onco Targets Ther. 2014; 7:1349-59.

NCBI and Uniprot Product Information

• NCBI GI #: 167857792
• NCBI GeneID: 5133
• NCBI Accession #: NP_005009.2
• NCBI GenBank Nucleotide #: NM_005018.2
• UniProt Accession #: Q15116; O00517; Q8IX89
• Molecular Weight: 31,647 Da
• NCBI Official Full Name: programmed cell death protein 1
• NCBI Official Synonym Full Names: programmed cell death 1
• NCBI Official Symbol: PDCD1
• NCBI Official Synonym Symbols: PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
• NCBI Protein Information: programmed cell death protein 1
• UniProt Protein Name: Programmed cell death protein 1
• UniProt Gene Name: PDCD1
• UniProt Synonym Gene Names: PD1; Protein PD-1; hPD-1

NCBI Description

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors.