Mouse anti-Human PD-1 Monoclonal Antibody | anti-PDCD1 antibody

PD-1 Antibody [10B3]

Cat. Num. AAA154534

• Gene Names: PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Protein A purified IgG1.
• Synonyms: PD-1; Monoclonal Antibody; PD-1 Antibody [10B3]; PD-1 Antibody; PD1; CD279; SLEB2; hPD-1; hPD-l; hSLE1; Programmed cell death protein 1; Protein PD-1; PDCD1; PDCD-1; anti-PDCD1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 10B3
• Purity/Purification: Protein A purified IgG1.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 288

• Applicable Applications for anti-PDCD1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: Western blot at 1 - 2 mug/mL.; Immunohistochemistry starting at 5 mug/mL.; Immunofluorescence start at 20 mug/mL.; Flow cytometry at 0.1 mug/ml.
• Conjugate: Unconjugated
• Immunogen: PD-1 antibody was raised against the extracellular domain of human PD-1.
• Buffer: PD-1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Species: Human
• Preparation and Storage: Store at 4 degree C for three months and -20 degree C, stable for up to one year.; As with all antibodies care should be taken to avoid repeated freeze thaw cycles; Antibodies should not be exposed to prolonged high temperatures.

Immunocytochemistry (ICC)

(Immunocytochemistry of PD-1 in transfected 293 cells with PD-1 [10B3] antibody at 10 μg/mL. Lower left: Immunocytochemistry in transfected 293 cells with control mouse IgG antibody at 10 μg/mL.)

Immunohistochemistry (IHC)

((A) Immunohistochemistry of PD-1 in human spleen tissue with PD-1 [10B3] antibody at 10 μg/mL. (B) Immunohistochemistry in human spleen tissue with control mouse IgG staining at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of PD-1 in human spleen tissue with PD-1 [10B3] antibody at 10 μg/mL.Green: PD1 Antibody [10B3]Blue: DAPI staining)

Flow Cytometry (FC/FACS)

(Flow cytometry analysis of PD-1 transfected 293 cells using PD-1 [10B3] antibody at 1 μg/ml. Blue: untransfected cells, Yellow: PD-1 transfected cells.)

Immunohistochemistry (IHC)

((A) Immunohistochemistry of PD-1 in human breast cancer tissue with PD-1 [10B3] antibody at 10 μg/mL. (B) Immunohistochemistry in human breast cancer tissue with control mouse IgG staining at 10 μg/mL.)

ELISA (EIA)

(Titration curve analysis of PD-1 mAbs to detect recombinant PD-1 in ELISA with abs at decreasing concentrations.)

Related Product Information for anti-PDCD1 antibody

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD-1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. PD-1 is thus one of a growing number of immune checkpoint proteins.

Product Categories/Family for anti-PDCD1 antibody

Apoptosis

References

1. Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90. 2. Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95. 3. Zhong X, Bai C, Gao W, et al. Suppression of expression and function of negative immune regulator PD-1 by certain pattern recognition and cytokine receptor signals associated with immune system danger. Int. Immunol. 2004; 16:1181-8. 4. Nishimura H, Nose M, Hiai H, et al. Development of lupus-like autoimmune diseases by the disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor. Immunity 1999; 11:141-51.

NCBI and Uniprot Product Information

• NCBI GI #: 145559515
• NCBI GeneID: 5133
• UniProt Accession #: Q15116; O00517; Q8IX89
• Molecular Weight: Predicted: 32 kDa; Observed: 38 kDa
• NCBI Official Full Name: Programmed cell death protein 1
• NCBI Official Synonym Full Names: programmed cell death 1
• NCBI Official Symbol: PDCD1
• NCBI Official Synonym Symbols: PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
• NCBI Protein Information: programmed cell death protein 1
• UniProt Protein Name: Programmed cell death protein 1
• Protein Family: Programmed cell death protein
• UniProt Gene Name: PDCD1
• UniProt Synonym Gene Names: PD1; Protein PD-1; hPD-1

NCBI Description

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

PDCD1: a single-pass type I membrane protein. A negative regulator of activated T cells, is upregulated in human and mouse 'exhausted' T cells during persistent viral infection. A marker of angioimmunoblastic T-cell lymphoma and germinal center associated T cells.

Protein type: Apoptosis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: plasma membrane

Molecular Function: protein binding; signal transducer activity

Biological Process: humoral immune response; multicellular organismal development; T cell costimulation

Disease: Systemic Lupus Erythematosus