Mouse anti-Human PD1 Monoclonal Antibody | anti-PD1 antibody

PD1 Antibody [5D3]

Cat. Num. AAA154625

• Gene Names: PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: PD-1 Antibody is supplied as protein A purified IgG1.
• Synonyms: PD1; Monoclonal Antibody; PD1 Antibody [5D3]; PD-1 Antibody: PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1; Programmed cell death protein 1; Protein PD-1; PDCD1; PDCD-1; anti-PD1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5D3
• Purity/Purification: PD-1 Antibody is supplied as protein A purified IgG1.
• Form/Format: Liquid; PD-1 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 288

• Applicable Applications for anti-PD1 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: PD-1 antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Flow cytometry at 1 ug/ml. Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.; Optimal dilutions for each application to be determined by the researcher.
• Immunogen: PD-1 antibody was raised against the extracellular domain of human PD-1.
• Conjugate: Unconjugated
• Preparation and Storage: Store at 4 degree C for three months and -20 degree C, stable for up to one year.; As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Immunocytochemistry (ICC)

(Immunocytochemistry of PD-1 in overexpressing HEK293 cells with PD-1 antibody at 10 μg/mL. Corner Box: Immunocytochemistry in transfected 293 cells with control mouse IgG antibody at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of PD-1 in in overexpressing HEK293 cells with PD-1 antibody at 20 μg/mL.Green: PD1 Antibody [5D3]Blue: DAPI staining)

Immunofluorescence (IF)

(Immunofluorescence of PD-1 in human lymph node tissue with PD-1 antibody at 20 μg/mL.Green: PD1 Antibody [10B3]Blue: DAPI staining)

Immunohistochemistry (IHC)

(Immunohistochemistry of PD-1 in human lymph node tissue with PD-1 antibody at 5 μg/mL. Corner Box: Immunocytochemistry in transfected 293 cells with control mouse IgG antibody at 10 μg/mL.)

Flow Cytometry (FC/FACS)

(Flow cytometry analysis of PD-1 in overexpressing HEK293 cells using PD-1 antibody at 1 μg/ml. Blue: untransfected cells, Yellow: PD-1 transfected cells.)

Related Product Information for anti-PD1 antibody

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD-1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. PD-1 is thus one of a growing number of immune checkpoint proteins.

Product Categories/Family for anti-PD1 antibody

Immunology

References

1. Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90. 2. Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95. 3. Zhong X, Bai C, Gao W, et al. Suppression of expression and function of negative immune regulator PD-1 by certain pattern recognition and cytokine receptor signals associated with immune system danger. Int. Immunol. 2004; 16:1181-8. 4. Nishimura H, Nose M, Hiai H, et al. Development of lupus-like autoimmune diseases by the disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor. Immunity 1999; 11:141-51.

NCBI and Uniprot Product Information

• NCBI GI #: 145559515
• NCBI GeneID: 5133
• UniProt Accession #: Q15116; O00517; Q8IX89
• Molecular Weight: 31,647 Da
• NCBI Official Full Name: Programmed cell death protein 1
• NCBI Official Synonym Full Names: programmed cell death 1
• NCBI Official Symbol: PDCD1
• NCBI Official Synonym Symbols: PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
• NCBI Protein Information: programmed cell death protein 1
• UniProt Protein Name: Programmed cell death protein 1
• UniProt Gene Name: PDCD1
• UniProt Synonym Gene Names: PD1; Protein PD-1; hPD-1

NCBI Description

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors.