Rabbit PDCD1 Polyclonal Antibody | anti-PDCD1 antibody

PDCD1, CT (Programmed Cell Death 1, PD-1, CD279)

Cat. Num. AAA6006513

• Gene Names: PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: PDCD1; Polyclonal Antibody; CT (Programmed Cell Death 1; PD-1; CD279); Anti -PDCD1; anti-PDCD1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PDCD1. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-PDCD1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 0.5-1ug/ml. See P3120-61A for control peptide.
• Immunogen: 16 amino acid peptide from near the carboxy terminus of human PDCD1 (GenBank accession no. Q15116)
• Positive Control: THP-1 cell lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of PDCD1 in THP-1 cell lysate using MBS6006513 at 1ug/ml in the (A) absence and (B) presence of blocking peptide.)

Immunofluorescence (IF)

(Immunofluorescence analysis of human brain tissue using MBS6006513 at 20ug/ml.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of human brain tissue using MBS6006513 at 2.4ug/ml.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of human tonsil tissue using MBS6006513 at 5ug/ml.)

Related Product Information for anti-PDCD1 antibody

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC) (1). T-cell activation is generally self-limited as activated T cells express receptors such as PDCD-1 that mediate inhibitory signals from the APC (2). PDCD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PDCD1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens (3). Evidence for this is seen in PDCD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases (4). Despite its predicted molecular weight, PDCD1 often migrates at higher molecular weight in SDS-PAGE.

Product Categories/Family for anti-PDCD1 antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 167857792
• NCBI GeneID: 5133
• NCBI Accession #: NP_005009.2
• NCBI GenBank Nucleotide #: NM_005018.2
• UniProt Accession #: Q15116; O00517; Q8IX89
• Molecular Weight: 31,647 Da
• NCBI Official Full Name: programmed cell death protein 1
• NCBI Official Synonym Full Names: programmed cell death 1
• NCBI Official Symbol: PDCD1
• NCBI Official Synonym Symbols: PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l
• NCBI Protein Information: programmed cell death protein 1; protein PD-1
• UniProt Protein Name: Programmed cell death protein 1
• Protein Family: Programmed cell death protein
• UniProt Gene Name: PDCD1
• UniProt Synonym Gene Names: PD1; Protein PD-1; hPD-1
• UniProt Entry Name: PDCD1_HUMAN

NCBI Description

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

PDCD1: a single-pass type I membrane protein. A negative regulator of activated T cells, is upregulated in human and mouse 'exhausted' T cells during persistent viral infection. A marker of angioimmunoblastic T-cell lymphoma and germinal center associated T cells.

Protein type: Apoptosis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; signal transducer activity

Biological Process: apoptosis; multicellular organismal development; T cell costimulation; signal transduction; humoral immune response

Disease: Systemic Lupus Erythematosus