Rabbit anti-Mouse P-Selectin Monoclonal Antibody | anti-SELP antibody

Recombinant Anti-P-Selectin Antibody, Rabbit Monoclonal

Cat. Num. AAA8112014

• Gene Names: Selp; Grmp; CD62P; LECAM3; PADGEM; GMP-140
• Reactivity: Mouse
• Applications: ELISA
• Purity: Protein A
• Synonyms: P-Selectin; Monoclonal Antibody; Recombinant Anti-P-Selectin Antibody; Rabbit Monoclonal; P-Selectin/CD62P/SELP Antibody; Rabbit MAb; selectin P (granule membrane protein 140kDa; antigen CD62); Anti-CD62P Antibody; Anti-GMP-140 Antibody; Anti-Grmp Antibody; Anti-LECAM3 Antibody; Anti-PADGEM Antibody; anti-SELP antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 001
• Specificity: Mouse P-Selectin/CD62P/SELP
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-SELP antibody: ELISA (EIA)
• Application Notes: ELISA: 1:5000-1:10,000
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Mouse P-Selectin/CD62P/SELP Protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse P-Selectin/CD62P/SELP (rM P-Selectin/CD62P/SELP).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-SELP antibody

P selectin (SELP) is a 140kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes.

References

Johnson-Tidey RR, et al. (1994) Increase in the adhesion molecule P-selectin in endothelium overlying atherosclerotic plaques. Coexpression with intercellular adhesion molecule-1. Am J Pathol. 144(5):952-61.Walcheck B, et al. (1996) Neutrophil-neutrophil interactions under hydrodynamic shear stress involve L-selectin and PSGL-1. A mechanism that amplifies initial leukocyte accumulation of P-selectin in vitro. J Clin Invest. 98(5):1081-7.Foreman KE, et al. (1994) C5a-induced expression of P-selectin in endothelial cells. J Clin Invest. 94(3):1147-55.

NCBI and Uniprot Product Information

• NCBI GI #: 266459
• NCBI GeneID: 20344
• UniProt Accession #: Q01102; Q32MF1
• Molecular Weight: 83,099 Da
• NCBI Official Full Name: P-selectin
• NCBI Official Synonym Full Names: selectin, platelet
• NCBI Official Symbol: Selp
• NCBI Official Synonym Symbols: Grmp; CD62P; LECAM3; PADGEM; GMP-140
• NCBI Protein Information: P-selectin; granule membrane protein 140; CD62 antigen-like family member P; leukocyte-endothelial cell adhesion molecule 3; platelet activation dependent granule-external membrane protein
• UniProt Protein Name: P-selectin
• Protein Family: P-selectin
• UniProt Gene Name: Selp
• UniProt Synonym Gene Names: Grmp; GMP-140; LECAM3; PADGEM
• UniProt Entry Name: LYAM3_MOUSE

Uniprot Description

SELP: Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the selectin/LECAM family.

Protein type: Membrane protein, integral; Cell adhesion

Cellular Component: nucleoplasm; extracellular space; platelet alpha granule membrane; membrane; cytoplasm; integral to membrane; external side of plasma membrane

Molecular Function: heparin binding; protein binding; lipopolysaccharide binding; carbohydrate binding; glycoprotein binding; calcium-dependent protein binding; sialic acid binding; fucose binding

Biological Process: heterophilic cell adhesion; positive regulation of phosphoinositide 3-kinase cascade; cell-cell adhesion; leukocyte adhesion; positive regulation of cell adhesion; regulation of integrin activation; positive regulation of leukocyte migration; regulation of cellular extravasation; blood vessel morphogenesis; cell adhesion; leukocyte tethering or rolling; inflammatory response; leukocyte migration