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Western Blot (WB) (Anti-MED8 rabbit polyclonal antibody at 1:500 dilution Lane A: HL60 Whole Cell Lysate Lane B: U87-MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:30 kDa)

Rabbit anti-Human MED8 Polyclonal Antibody | anti-MED8 antibody

Anti-MED8 Antibody, Rabbit Polyclonal

Reactivity
Human
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
MED8; Polyclonal Antibody; Anti-MED8 Antibody; Rabbit Polyclonal; MED8 Antibody; Rabbit PAb; Antigen Affinity Purified; mediator complex subunit 8; Anti-ARC32 Antibody; anti-MED8 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human MED8
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-MED8 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
E Coli-derived Human MED8 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human MED8 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-MED8 rabbit polyclonal antibody at 1:500 dilution Lane A: HL60 Whole Cell Lysate Lane B: U87-MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:30 kDa)

Western Blot (WB) (Anti-MED8 rabbit polyclonal antibody at 1:500 dilution Lane A: HL60 Whole Cell Lysate Lane B: U87-MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:30 kDa)
Related Product Information for anti-MED8 antibody
Med8, a subunit of mediator complex, has proved to possess crucial functions in many organisms from yeast to human. Med8 protein is a regulator that specifically binds to upstream activating sequences (UASs) of SUC2 promoter, downstream repressing sequences (DRSs) of the HXK2 gene, and to the carboxy-terminal domain of the RNA polymerase II. In the plant, the med8 mutant of Arabidopsis thaliana displayed delayed anthesis and an increased number of leaves during the vegetative period. A loss-of-function mutation in the Mediator complex subunit 8 (MED8) causes small flowers as a result of reduced cell expansion. Analysis of the med8 med25-2 double mutant reveals that MED8 acts independently of MED25 to regulate cell expansion and organ growth. The MED8 subunit of the A. thaliana Mediator regulates plant immunity to B. cinerea through interacting with the transcription factor FAMA, which was discovered to be a key component in B. cinerea resistance.
References
Xu R, et al. (2012) The mediator complex subunit 8 regulates organ size in arabidopsis thaliana. Plant Signal Behav 7 (2): 182-183.Wang F, et al. (2011) Knockdown of ntmed8, a med8-like gene, causes abnormal development of vegetative and floral organs in tobacco (nicotiana tabacum l.). Plant Cell Rep 30 (11): 2117-2129.Moreno-Herrero F, et al. (1999) Analysis by atomic force microscopy of med8 binding to cis-acting regulatory elements of the suc2 and hxk2 genes of saccharomyces cerevisiae. FEBS Lett 459 (3): 427-432.Li X, et al. (2018) The arabidopsis thaliana mediator subunit med8 regulates plant immunity to botrytis cinerea through interacting with the basic helix-loop-helix (bhlh) transcription factor fama. PLoS One 13 (3): e0193458.

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Product Notes

The MED8 (Catalog #AAA8108268) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MED8 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MED8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the MED8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MED8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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