SELP / selectin P / P-selectin Recombinant Protein | SELP recombinant protein

Recombinant Mouse SELP / selectin P / P-selectin Protein (His tag)

Cat. Num. AAA2546809

• Gene Names: Selp; Grmp; CD62P; LECAM3; PADGEM; GMP-140
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: SELP / selectin P / P-selectin; Recombinant Mouse SELP / selectin P / P-selectin Protein (His tag); SELP; Grmp; SELP recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 768

• Application Notes: The recombinant mouse SELP comprises 679 amino acids and has a predicted molecular mass of 74 kDa. The apparent molecular mass of the protein is approximately 116 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Trp 42
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for SELP recombinant protein

Background: P selectin (SELP) is a 140kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interacton of activated endothelial cells or platelets with leukocytes.

Description: A DNA sequence encoding the mouse SELP (Q01102) (Met1-Ala709) was expressed with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 6755456
• NCBI GeneID: 20344
• NCBI Accession #: NP_035477.1
• NCBI GenBank Nucleotide #: NM_011347.2
• UniProt Accession #: Q01102; Q32MF1
• Molecular Weight: 83,099 Da
• NCBI Official Full Name: P-selectin
• NCBI Official Synonym Full Names: selectin, platelet
• NCBI Official Symbol: Selp
• NCBI Official Synonym Symbols: Grmp; CD62P; LECAM3; PADGEM; GMP-140
• NCBI Protein Information: P-selectin
• UniProt Protein Name: P-selectin
• Protein Family: P-selectin
• UniProt Gene Name: Selp
• UniProt Synonym Gene Names: Grmp; GMP-140; LECAM3; PADGEM

Uniprot Description

SELP: Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the selectin/LECAM family.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1 H2.2|1 71.42 cM

Cellular Component: cytoplasm; external side of plasma membrane; extracellular space; membrane; nucleoplasm; platelet alpha granule membrane

Molecular Function: calcium-dependent protein binding; fucose binding; glycoprotein binding; heparin binding; lipopolysaccharide binding; protein binding; sialic acid binding

Biological Process: cell-cell adhesion; heterophilic cell adhesion; inflammatory response; leukocyte adhesion; leukocyte migration; leukocyte tethering or rolling; positive regulation of cell adhesion; positive regulation of leukocyte migration; positive regulation of phosphoinositide 3-kinase cascade; regulation of cellular extravasation; regulation of integrin activation