P-Selectin Active Protein | SELP active protein

P-Selectin Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122965

• Gene Names: Selp; Grmp; CD62P; LECAM3; PADGEM; GMP-140
• Purity: >95% as determined by SDS-PAGE
• Synonyms: P-Selectin; P-Selectin Protein; Mouse; Recombinant (His Tag); Mouse SELP/selectin P/P-selectin Protein (His Tag); CD62P Protein; GMP-140 Protein; Grmp Protein; LECAM3 Protein; PADGEM Protein; selectin P (granule membrane protein 140kDa; antigen CD62); SELP active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala709

• Species: Mouse
• Activity: Measured by the ability of the immobilized protein to support the adhesion of U937 cells. When 5 x 10E4 cells/well are added to SELP-coated plates (5ug/mL and 100uL/well), approximately >60% cells will adhere specifically after 60 minutes at 37 degree C.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Trp 42
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse SELP (Q01102)(Met1-Ala709) was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for SELP active protein

Background: P selectin (SELP) is a 140kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes.

References

Johnson-Tidey RR, et al. (1994) Increase in the adhesion molecule P-selectin in endothelium overlying atherosclerotic plaques. Coexpression with intercellular adhesion molecule-1. Am J Pathol. 144(5):952-61.Walcheck B, et al. (1996) Neutrophil-neutrophil interactions under hydrodynamic shear stress involve L-selectin and PSGL-1. A mechanism that amplifies initial leukocyte accumulation of P-selectin in vitro. J Clin Invest. 98(5):1081-7.Foreman KE, et al. (1994) C5a-induced expression of P-selectin in endothelial cells. J Clin Invest. 94(3):1147-55.

NCBI and Uniprot Product Information

• NCBI GI #: 266459
• NCBI GeneID: 20344
• UniProt Accession #: Q01102; Q32MF1
• Molecular Weight: 83,099 Da
• NCBI Official Full Name: P-selectin
• NCBI Official Synonym Full Names: selectin, platelet
• NCBI Official Symbol: Selp
• NCBI Official Synonym Symbols: Grmp; CD62P; LECAM3; PADGEM; GMP-140
• NCBI Protein Information: P-selectin; granule membrane protein 140; CD62 antigen-like family member P; leukocyte-endothelial cell adhesion molecule 3; platelet activation dependent granule-external membrane protein
• UniProt Protein Name: P-selectin
• Protein Family: P-selectin
• UniProt Gene Name: Selp
• UniProt Synonym Gene Names: Grmp; GMP-140; LECAM3; PADGEM
• UniProt Entry Name: LYAM3_MOUSE

Uniprot Description

SELP: Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the selectin/LECAM family.

Protein type: Membrane protein, integral; Cell adhesion

Cellular Component: nucleoplasm; extracellular space; platelet alpha granule membrane; membrane; cytoplasm; integral to membrane; external side of plasma membrane

Molecular Function: heparin binding; protein binding; lipopolysaccharide binding; carbohydrate binding; glycoprotein binding; calcium-dependent protein binding; sialic acid binding; fucose binding

Biological Process: heterophilic cell adhesion; positive regulation of phosphoinositide 3-kinase cascade; cell-cell adhesion; leukocyte adhesion; positive regulation of cell adhesion; regulation of integrin activation; positive regulation of leukocyte migration; regulation of cellular extravasation; blood vessel morphogenesis; cell adhesion; leukocyte tethering or rolling; inflammatory response; leukocyte migration