Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse L-Selectin (SELL) Standard Curve.)
Mouse L-Selectin (SELL) ELISA Kit | SELL elisa kit
Mouse L-Selectin (SELL) ELISA Kit
Gene Names
Sell; Lnhr; CD62L; Ly-22; Lyam1; Ly-m22; Lyam-1; LECAM-1; AI528707; L-selectin
Reactivity
Mouse
Synonyms
L-Selectin (SELL); Mouse L-Selectin (SELL) ELISA Kit; CD62L; LAM1; LECAM1; LEU8; LNHR; LSEL; LYAM1; PLNHR; TQ1; CD62 antigen-like family member L; gp90-MEL; leukocyte surface antigen Leu-8; leukocyte-endothelial cell adhesion molecule 1; lymph node homing; SELL elisa kit
Reactivity
Mouse
Specificity
Mouse L-Selectin (SELL) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SELL. No significant cross-reactivity or interference between Mouse SELL and analogues was observed.
Sequence Length
372
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse L-Selectin (SELL) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse L-Selectin (SELL) Standard Curve.)
Related Product Information for SELL elisa kit
Description: This Mouse L-Selectin (SELL) ELISA Kit employs a two-site sandwich ELISA to quantitate SELL in samples. An antibody specific for SELL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SELL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SELL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SELL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognise sialylated carbohydrate groups. It is cleaved by ADAM17.The protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. L-selectin acts as a "homing receptor" for leukocytes to enter tissues at the site of inflammation. Ligands present on endothelial cells will bind to leukocytes expressing L-selectin, causing them to become localised at that point. The receptor is also found on the cell surfaces of "naive" T cells, which have not yet encountered their specific antigen. This surface expression is lost after the cells are activated.
Background: L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognise sialylated carbohydrate groups. It is cleaved by ADAM17.The protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. L-selectin acts as a "homing receptor" for leukocytes to enter tissues at the site of inflammation. Ligands present on endothelial cells will bind to leukocytes expressing L-selectin, causing them to become localised at that point. The receptor is also found on the cell surfaces of "naive" T cells, which have not yet encountered their specific antigen. This surface expression is lost after the cells are activated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
L-selectin
NCBI Official Synonym Full Names
selectin, lymphocyte
NCBI Official Symbol
Sell
NCBI Official Synonym Symbols
Lnhr; CD62L; Ly-22; Lyam1; Ly-m22; Lyam-1; LECAM-1; AI528707; L-selectin
NCBI Protein Information
L-selectin
UniProt Protein Name
L-selectin
Protein Family
UniProt Gene Name
Sell
UniProt Synonym Gene Names
Lnhr; Ly-22; Ly22; LAM-1; LECAM1; Ly-22
UniProt Entry Name
LYAM1_MOUSE
Uniprot Description
SELL: Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. Belongs to the selectin/LECAM family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Cellular Component: cell surface; membrane; integral to membrane; plasma membrane; external side of plasma membrane
Molecular Function: protein binding; protease binding; cell adhesion molecule binding; carbohydrate binding; glycolipid binding
Biological Process: regulation of apoptosis; cell adhesion; response to ATP
Research Articles on SELL
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Product Notes
The Mouse SELL sell (Catalog #AAA9717072) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717072 ELISA Kit recognizes Mouse SELL. It is sometimes possible for the material contained within the vial of "L-Selectin (SELL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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