Mouse Nuclear Antigen (HNA) (Human Cell Marker) Monoclonal Antibody

Human Nuclear Antigen (HNA) (Human Cell Marker)

Cat. Num. AAA4381509

• Reactivity: Human, Non-Human primates; Does not react with Mouse, Rat or Chicken.
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Purified Ab conjugated to CF488
• Synonyms: Nuclear Antigen (HNA) (Human Cell Marker); Monoclonal Antibody; Human Nuclear Antigen (HNA) (Human Cell Marker); HNA; HuNu; anti-Nuclear Antigen (HNA) (Human Cell Marker) antibody

• Host: Mouse
• Reactivity: Human, Non-Human primates; Does not react with Mouse, Rat or Chicken.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: 235-1
• Specificity: This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
• Purity/Purification: Purified Ab conjugated to CF488
• Form/Format: Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.

• Applicable Applications for anti-Nuclear Antigen (HNA) (Human Cell Marker) antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: FC/FACS: 5ul per test per one million cells or 5ul per 100ul of whole blood; IF: 1:50-1:100
• Positive Control: All human cells. Tissues.
• Immunogen: Nuclei of human myeloid leukemia biopsy cells
• Cellular Localization: Nuclei
• Preparation and Storage: Antibody with azide: store at 4 to 8 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Flow Cytometry (FC/FACS)

(Flow Cytometry of Human Nuclear Ag (HNA) on 293T cells. Black: cells alone; Grey: Isotype Control; Green: CF488-labeled HNA Monoclonal Antibody (235-1).)

References

1. Glaser R, Lu MM, Narula N, Epstein JA (2002) Smooth muscle cells, but not myocytes, of host origin in transplanted human hearts. Circulation Jul 2;106(1):17-9. Parthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's diseaseParthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's disease Parthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's disease. 2. Rosario Sanchez-Pernaute et. al. Parthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's disease. Brain. 2008; 131(8): 2127-2139.