Mouse Nucleolar Antigen Monoclonal Antibody

Human Nucleolar Antigen (Marker For Human Cells) Mouse Monoclonal Antibody

Cat. Num. AAA438004

• Reactivity: Human.; Does not react with Mouse, Rat and Cow
• Applications: Immunofluorescence, Immunocytochemistry, Immunohistochemistry
• Synonyms: Nucleolar Antigen; Monoclonal Antibody; Human Nucleolar Antigen (Marker For Human Cells) Mouse Monoclonal Antibody; anti-Nucleolar Antigen antibody

• Host: Mouse
• Reactivity: Human.; Does not react with Mouse, Rat and Cow
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: NM95
• Specificity: This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb NM95 recognizes an antigen associated with the nucleoli in human cells. It can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

• Applicable Applications for anti-Nucleolar Antigen antibody: Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin
• Application Notes: Immunofluorescence (0.5-1.0ug/ml); Immunocytochemistry (Acetone-fixed cells) (0.5-1.0ug/ml for 30 minutes at RT); Immunohistochemistry (Formalin-paraffin) (0.25-0.5ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Nucleoli
• Immunogen: Nuclei of myeloid leukemia biopsy cells
• Positive Control: Any human cells. Tonsil
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Skin stained with Nucleolar Monoclonal Antibody (NM95).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Tonsil stained with Nucleolar Monoclonal Antibody (NM95).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with AF488 Conjugate of Nucleolar Monoclonal Antibody (NM95).)

Flow Cytometry (FC/FACS

(Flow Cytometry of Human Nucleolar Ag on 293T Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled human Nucleolar Monoclonal Antibody (NM95).)

References

Epstein, A.L. and Clevenger, C.V., Identification of nuclear antigens in human cells by immunofluorescence, immunoelectron microscopy, and immuno-biochemical methods using monoclonal antibodies. In Progress on nonhistone protein research, Vol. 1, Isaac Bekhor, ed., 1985, CRC Press, Boca Raton, FL, pp 117-137