Western Blot (WB)
((0.2ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat SIRT3 Polyclonal Antibody | anti-SIRT3 antibody
Goat anti-SIRT3 Antibody
Western Blot: Approx 28kDa band observed in Human Liver lysates (calculated MW of 28.6kDa according to NP_001017524.1). In transfected HEK293 transiently expressing SIRT3 bands of approx.35kDa and 48kDa are observed. These bands are not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3ug/ml.
Western Blot (WB)
((0.2ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.2ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(HEK293 overexpressing SIRT3 (RC200190) and probed (mock transfection in first lane), tested by Origene.)
Western Blot (WB)
(HEK293 overexpressing SIRT3 (RC200190) and probed (mock transfection in first lane), tested by Origene.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
SIRT3: a soluble mitochondrial NAD-dependent protein deacetylase. Can deacetylate and thereby activate a central metabolic regulator in the mitochondrial matrix, glutamate dehydrogenase. Mitochondrial proteins are hyperacetylated in SIRT3-deficient mice, but not in SIRT4 or SIRT5 deficient mice. SIRT3-deficient mice have no obvious metabolic phenotype, and the process of adaptive thermogenesis is normal. Can deacetylate and activate isocitrate dehydrogenase 2, an enzyme that participates in regulation of the citric acid cycle and the regeneration of antioxidants. Inhibited by nicotinamide, but not by trichostatin A. Its unprocessed form is enzymatically inactive. It is processed by mitochondrial processing peptidase (MPP) in the mitochondrial matrix to give an enzymatically active 28 kDa product.
Protein type: Mitochondrial; Deacetylase; EC 3.5.1.-
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: mitochondrial inner membrane; mitochondrial matrix; mitochondrion
Molecular Function: NAD+ ADP-ribosyltransferase activity; NAD-dependent histone deacetylase activity (H3-K14 specific); protein binding; zinc ion binding
Biological Process: aerobic respiration; mitochondrion organization and biogenesis; protein amino acid ADP-ribosylation; protein amino acid deacetylation
Research Articles on SIRT3
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Product Notes
The SIRT3 sirt3 (Catalog #AAA421123) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-SIRT3 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's SIRT3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 28kDa band observed in Human Liver lysates (calculated MW of 28.6kDa according to NP_001017524.1). In transfected HEK293 transiently expressing SIRT3 bands of approx.35kDa and 48kDa are observed. These bands are not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the SIRT3 sirt3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QRETGKLDGP DK. It is sometimes possible for the material contained within the vial of "SIRT3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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