Immunofluorescence (IF)
(Immunofluorescent analysis of NPM1 in WiDr cells, stained with DAPI (Blue) for nucleus staining and NPM1 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse NPM1 Monoclonal Antibody | anti-NPM1 antibody
NPM1 antibody
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Purified by Protein A affinity chromatography
Synonyms
NPM1; Monoclonal Antibody; NPM1 antibody; Mouse Monoclonal NPM1 antibody; Monoclonal NPM1 antibody; Anti-NPM1 antibody; Nucleophosmin antibody; B23 antibody; NPM antibody; anti-NPM1 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
AT23F1
Purity/Purification
Purified by Protein A affinity chromatography
Form/Format
Liquid form in Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide and 10% Glycerol
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-NPM1 antibody
ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)
Application Notes
WB: 1:1000
ICC: 1:200
IF: 1:200
ICC: 1:200
IF: 1:200
Immunogen
NPM1 antibody was raised in Mouse using recombinant human NPM1(1-294aa) purified from E Coli as the immunogen
Preparation and Storage
Store at 4 degree C for short term storage. For long term storage, aliquot and store at-20 degree C.
Avoid repeat freeze/thaw cycles.
Avoid repeat freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescent analysis of NPM1 in WiDr cells, stained with DAPI (Blue) for nucleus staining and NPM1 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF)
(Immunofluorescent analysis of NPM1 in WiDr cells, stained with DAPI (Blue) for nucleus staining and NPM1 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Related Product Information for anti-NPM1 antibody
NPM1 is associated with nucleolar ribonucleoprotein structures and bind single-stranded and double-stranded nucleic acids, but it binds preferentially G-Quadruplex forming nucleic acids. It is involved in the biogenesis of ribosomes and may assist small basic proteins in their transport to the nucleolus. Its regulation through SUMOylation (by SENP3 and SENP5) is another facet of the proteins's regulation and cellular functions.
Product Categories/Family for anti-NPM1 antibody
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Product Notes
The NPM1 (Catalog #AAA5310741) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NPM1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB). WB: 1:1000 ICC: 1:200 IF: 1:200. Researchers should empirically determine the suitability of the NPM1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NPM1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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