Immunofluorescence (IF)
(Immunofluorescent analysis of GPC4 in 293T cells line, stained with DAPI (Blue) for nucleus staining and GPC4 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse GPC4 Monoclonal Antibody | anti-GPC4 antibody
GPC4 antibody
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Purified by Protein A affinity chromatography
Synonyms
GPC4; Monoclonal Antibody; GPC4 antibody; Mouse Monoclonal GPC4 antibody; Monoclonal GPC4 antibody; Anti-GPC4 antibody; Glypican 4 antibody; dJ900E8.1 antibody; glypican proteoglycan 4 antibody; anti-GPC4 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a kappa
Clone Number
AT51E3
Purity/Purification
Purified by Protein A affinity chromatography
Form/Format
Liquid form in Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide and 10% Glycerol
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-GPC4 antibody
ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)
Application Notes
WB: 1:1000
ICC: 1:200
IF: 1:200
ICC: 1:200
IF: 1:200
Immunogen
GPC4 antibody was raised in Mouse using recombinant human GPC4 (401-529aa) purified from E Coli as the immunogen
Preparation and Storage
Store at 4 degree C for short term storage. For long term storage, aliquot and store at-20 degree C.
Avoid repeat freeze/thaw cycles.
Avoid repeat freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescent analysis of GPC4 in 293T cells line, stained with DAPI (Blue) for nucleus staining and GPC4 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF)
(Immunofluorescent analysis of GPC4 in 293T cells line, stained with DAPI (Blue) for nucleus staining and GPC4 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Related Product Information for anti-GPC4 antibody
The Glypican (GPC) family of cell surface heparan sulfate proteoglycans is expressed in a tissue specific and developmentally regulated fashion. Expression of Glypican 4 has been reported in the mouse brain at embryonic day 10 and later stages and low during early tubule formation but is up-regulated in mature tubules. Accordingly it is not detected in the midline of the embryo at the initial stage of neural tube closure, suggesting that GPC4 is unlikely to play an essential role in convergent extension in the mouse.
Product Categories/Family for anti-GPC4 antibody
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Product Notes
The GPC4 (Catalog #AAA5310742) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GPC4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB). WB: 1:1000 ICC: 1:200 IF: 1:200. Researchers should empirically determine the suitability of the GPC4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPC4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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