Immunofluorescence (IF)
(Immunofluorescent analysis of GPC4 in 293T cells line, stained with DAPI (Blue) for nucleus staining and GPC4 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488)
Mouse KRT20 Monoclonal Antibody | anti-KRT20 antibody
KRT20 antibody
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Purified by Protein A affinity chromatography
Synonyms
KRT20; Monoclonal Antibody; KRT20 antibody; Mouse Monoclonal KRT20 antibody; Monoclonal KRT20 antibody; Anti-KRT20 antibody; Keratin antibody; type I cytoskeletal 20 antibody; CD20 antibody; CK-20 antibody; CK20 antibody; K20 antibody; KRT21 antibody; anti-KRT20 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a kappa
Clone Number
AT10D8
Purity/Purification
Purified by Protein A affinity chromatography
Form/Format
Liquid form in Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide and 10% Glycerol
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-KRT20 antibody
ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)
Application Notes
WB: 1:3000
ICC: 1:200
IF: 1:200
ICC: 1:200
IF: 1:200
Immunogen
KRT20 antibody was raised in Mouse using recombinant human KRT20 (1-424aa) purified from E Coli as the immunogen
Preparation and Storage
Store at 4 degree C for short term storage. For long term storage, aliquot and store at-20 degree C.
Avoid repeat freeze/thaw cycles.
Avoid repeat freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescent analysis of GPC4 in 293T cells line, stained with DAPI (Blue) for nucleus staining and GPC4 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488)
Immunofluorescence (IF)
(Immunofluorescent analysis of GPC4 in 293T cells line, stained with DAPI (Blue) for nucleus staining and GPC4 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488)
Related Product Information for anti-KRT20 antibody
Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa. In immunohistochemistry, antibodies to KRT20 can be used to identify a range of adenocarcinoma arising from epithelia that normally contain the KRT20 protein. For example, the protein is commonly found in colorectal cancer, transitional cell carcinomas and in Merkel cell carcinoma, but is absent in lung cancer, prostate cancer, and non-mucinous ovarian cancer. It is often used in combination with antibodies to CK7 to distinguish different types of glandular tumor.
Product Categories/Family for anti-KRT20 antibody
Similar Products
Product Notes
The KRT20 (Catalog #AAA5310734) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's KRT20 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB). WB: 1:3000 ICC: 1:200 IF: 1:200. Researchers should empirically determine the suitability of the KRT20 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KRT20, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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