Mouse anti-Human CytokeRatin 16 / KeRatin K16 Monoclonal Antibody

Mouse anti CytokeRatin 16 / KeRatin K16

Cat. Num. AAA570081

• Reactivity: Human
• Applications: Immunohistochemistry, Immunohistochemistry, Western Blot
• Synonyms: CytokeRatin 16 / KeRatin K16; Monoclonal Antibody; Mouse anti CytokeRatin 16 / KeRatin K16; anti-CytokeRatin 16 / KeRatin K16 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: LL025
• Specificity: KeRatin 16 is specifically found in hyperprolifeRative squamous epithelium such as psoriatic lesions and squamous cell carcinomas, e.g. of the lung. It is expressed in suprabasal keRatinocytes of wounded epidermis, a situation of high cell turnover. LL025 reactivity is negative in, e.g. normal skin, breast and lung tissue and non-invasive breast carcinoma. However, 10% of the invasive breast carcinomas tested were diffusely or focally positive with this antibody.
• Form/Format: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

• Applicable Applications for anti-CytokeRatin 16 / KeRatin K16 antibody: Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB)
• Application Notes: LL025 is suitable for immunoblotting (Western blot) and for immunohistochemistry on frozen tissue and paraffin embedded material. Optimal antibody dilution should be determined by titRation; 1:25 - 1:50 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent. For use in paraffin embedded tissue sections high pressure retrieval at pH 8,0 is recommended.
• Source Note: LL025 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 murine myeloma cells with spleen cells from a Mouse immunized with a synthetic peptide corresponding to an amino acid sequence of the carboxyl terminus of Human keRatin 16.
• Preparation and Storage: Store at 4 degree C, or in small aliquots at -20 degree C.

Testing Data

(Figure 1: Immunohistochemistry on frozen section of human oesophagus )

Related Product Information for anti-CytokeRatin 16 / KeRatin K16 antibody

CytokeRatins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual cytokeRatin polypeptides are numbered 1 to 20. The various epithelia in the Human body usually express cytokeRatins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. CytokeRatin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeRatin antibodies are not only of assistance in the differential identification of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Product Categories/Family for anti-CytokeRatin 16 / KeRatin K16 antibody

Cytoskeletal; Cell differentiation; Cytoskeleton

References

Wetzels, R.H.W., Kuijpers H.J.H., Lane, E.B., Leigh, I.M., Troyanovsky, S.M., Holland, R., van Haelst, U.J.G.M., Ramaekers, F.C.S. (1991). Basal cell-specific and hyperprolifeRation-related keRatins in Human breast cancer. Am. J. Pathol. 138, 751-63. Porter, R.M., Lunny, D.P., Ogden, P.H., Morley, S.M., McLean, W.H., Evans, A., Harrison, D.L., Rugg, E.L., Lane, E.B. (2000). K15 expression implies lateral differentiation within stRatified epithelial basal cells. Lab Invest. 80, 1701-10. Ahenkorah, A., Hottor, B., Byrne, S., Bosio, P. and Ockleford, C.D. (2008). Immunofluorescence confocal laser scanning microscopy and immuno-electron microscopic identifiCation of keRatins in Human materno-foetal interaction zone. J. Cell. Mol. Med. 13, 735-48. van Lingen, R.G., Poll, M.K.P., Seyger, M.M.B., de Jong, E.M.G.J., van de Kerkhof, P.C.M., van Erp, P.E.J. (2008). Distribution of peptidyl-peptidase IV on keRatinocytes in the margin zone of a psoriatic lesion: a comparison with hyperprolifeRation and aberrant differentiation markers. Arch Dermatol Res 300, 561-67. Poschmann, G., Sitek, B., Sipos, B., Ulrich, A., Wiese, S., Stephan, C., Warscheid, B., Klöppel, G., Vander Borght, A., Ramaekers, F.C.S., Meyer, E. and Stühler, K. (2009). IdentifiCation of proteomic differences between squamous cell carcinoma of the lung and bronchial epithelium. Molecular & Cellular proteomics 8, 1105-16.