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Western Blot (WB)
(Western Blot (WB) analysis of NeuN Rabbit mAb at 1:1000 dilutionLane1: The brain tissue lysate of Human ( NeuN is expressed as two alternate transcripts with apparent SDS-PAGE molecular weight 46 and 48kDa))
Rabbit NeuN Monoclonal Antibody | anti-NeuN antibody
NeuN Rabbit Monoclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Protein A affinity purified
Synonyms
NeuN; Monoclonal Antibody; NeuN Rabbit Monoclonal Antibody; RNA binding protein fox-1 homolog 3; Fox-1 homolog C; RBFOX3; Fox-3; Fox3; Neuronal Marker; anti-NeuN antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG
Specificity
This antibody detects endogenous levels of NeuN and does not cross-react with related proteins.
Purity/Purification
Protein A affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Applicable Applications for anti-NeuN antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-1:2000
ICC: 1:50-1:20
IF: 1:50-1:200
IHC: 1:200-1:500
FC/FACS: 1:50-1:100
ICC: 1:50-1:20
IF: 1:50-1:200
IHC: 1:200-1:500
FC/FACS: 1:50-1:100
Immunogen
Recombinant antibody.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of NeuN Rabbit mAb at 1:1000 dilutionLane1: The brain tissue lysate of Human ( NeuN is expressed as two alternate transcripts with apparent SDS-PAGE molecular weight 46 and 48kDa))
Western Blot (WB)
(Western Blot (WB) analysis of NeuN Rabbit mAb at 1:1000 dilutionLane1: The brain tissue lysate of Human ( NeuN is expressed as two alternate transcripts with apparent SDS-PAGE molecular weight 46 and 48kDa))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NeuN antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NeuN antibody. Counter stained with hematoxylin.)
Immunocytochemistry (ICC)
(ICC staining NeuN in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS)
Immunocytochemistry (ICC)
(ICC staining NeuN in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-NeuN antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-NeuN antibody. Counter stained with hematoxylin.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of SH-SY-5Y cells with NeuN antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of SH-SY-5Y cells with NeuN antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
Related Product Information for anti-NeuN antibody
Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, and dentate nucleus neurons. This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS-analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator. Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
Predicted Band Size: 34KDa
Observed Band Size: 46, 48KDa
Observed Band Size: 46, 48KDa
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Product Notes
The NeuN (Catalog #AAA3014062) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NeuN Rabbit Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NeuN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:1000-1:2000 ICC: 1:50-1:20 IF: 1:50-1:200 IHC: 1:200-1:500 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the NeuN for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NeuN, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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