SDS-Page
Phospholipase A2, Group IID (PLA2G2D) Recombinant Protein | PLA2G2D recombinant protein
Recombinant Phospholipase A2, Group IID (PLA2G2D)
Gene Names
Pla2g2d; mGIID; Splash
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Phospholipase A2; Group IID (PLA2G2D); Recombinant Phospholipase A2; PLA2G2D recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-LLNLNKMVTH MTGKKAFFSY WPYGCHCGLG GKGQPKDATD WCCQKHDCCY AHLKIDGCKS LTDNYKYSIS QGTIQCSDNG SWCERQLCAC DKEVALCLKQ NLDSYNKRLR YYWRPRCKGK TPAC
MGHHHHHHSGSEF-LLNLNKMVTH MTGKKAFFSY WPYGCHCGLG GKGQPKDATD WCCQKHDCCY AHLKIDGCKS LTDNYKYSIS QGTIQCSDNG SWCERQLCAC DKEVALCLKQ NLDSYNKRLR YYWRPRCKGK TPAC
Sequence Length
144
Applicable Applications for PLA2G2D recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Mus musculus (Mouse)
Expression System
Prokaryotic expression
Residues
Leu21~Cys144 (Accession # Q9WVF6) with N-terminal His-Tag
Subcellular Location
Secreted. Cytoplasm
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Predicted Isoelectric Point
8.8
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15.7kDa
NCBI Official Full Name
group IID secretory phospholipase A2
NCBI Official Synonym Full Names
phospholipase A2, group IID
NCBI Official Symbol
Pla2g2d
NCBI Official Synonym Symbols
mGIID; Splash
NCBI Protein Information
group IID secretory phospholipase A2; PLA2IID; sPLA2-IID; GIID sPLA2; phosphatidylcholine 2-acylhydrolase 2D; secretory-type PLA, stroma-associated homolog
UniProt Protein Name
Group IID secretory phospholipase A2
Protein Family
UniProt Gene Name
Pla2g2d
UniProt Synonym Gene Names
Pla2a2; Splash; GIID sPLA2; sPLA2-IID
UniProt Entry Name
PA2GD_MOUSE
Uniprot Description
PLA2G2D: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. L-alpha-1-palmitoyl-2- linoleoyl phosphatidylethanolamine is more efficiently hydrolyzed than the other phospholipids examined. Belongs to the phospholipase A2 family.
Protein type: Lipid Metabolism - ether lipid; Secreted, signal peptide; Phospholipase; Secreted; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - arachidonic acid; Lipid Metabolism - linoleic acid; EC 3.1.1.4; Lipid Metabolism - alpha-linolenic acid
Cellular Component: cytoplasm; extracellular region
Molecular Function: phospholipase A2 activity; hydrolase activity; metal ion binding; calcium ion binding
Biological Process: phospholipid metabolic process; lipid metabolic process; lipid catabolic process
Research Articles on PLA2G2D
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Product Notes
The PLA2G2D pla2g2d (Catalog #AAA2009559) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Phospholipase A2, Group IID (PLA2G2D) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the PLA2G2D pla2g2d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSG SEF-LLNLNK MVTH MTGKKAFFSY WPYGCHCGLG GKGQPKDATD WCCQKHDCCY AHLKIDGCKS LTDNYKYSIS QGTIQCSDNG SWCERQLCAC DKEVALCLKQ NLDSYNKRLR YYWRPRCKGK TPAC. It is sometimes possible for the material contained within the vial of "Phospholipase A2, Group IID (PLA2G2D), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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