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Western Blot (WB) (Western blot analysis of extracts of various celllines, using NeuN antibody.)

NeuN Antibody | anti-NeuN antibody

NeuN Antibody

Gene Names
RBFOX3; FOX3; NEUN; FOX-3; HRNBP3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified
Synonyms
NeuN; NeuN Antibody; FLJ56884; FLJ58356; FOX3; HRNBP3; RBFOX3; NEUN; FOX-3; anti-NeuN antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse, Rat
Specificity
NeuN Antibody detects endogenous levels of total NeuN
Purity/Purification
Immunogen affinity purified
Form/Format
In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
312
Applicable Applications for anti-NeuN antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
Immunogen
Recombinant protein of human NeuN
Preparation and Storage
Store at-20 degree C for 1 year

Western Blot (WB)

(Western blot analysis of extracts of various celllines, using NeuN antibody.)

Western Blot (WB) (Western blot analysis of extracts of various celllines, using NeuN antibody.)
Related Product Information for anti-NeuN antibody
NeuN, also named as FOX3 and RBFOX3, is neuronal-specific nuclear protein with MW 38-50kd(~48kd). It is one of a family of 3 mammalian Fox homologues. FOX3(Or NeuN) functions in RNA-binding protein that regulates alternative splicing events.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34kDa
NCBI Official Full Name
RNA binding protein fox-1 homolog 3 isoform 1
NCBI Official Synonym Full Names
RNA binding fox-1 homolog 3
NCBI Official Symbol
RBFOX3
NCBI Official Synonym Symbols
FOX3; NEUN; FOX-3; HRNBP3
NCBI Protein Information
RNA binding protein fox-1 homolog 3
UniProt Protein Name
RNA binding protein fox-1 homolog 3
UniProt Gene Name
RBFOX3
UniProt Synonym Gene Names
NeuN antigen

NCBI Description

This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2017]

Uniprot Description

Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD).

Research Articles on NeuN

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Product Notes

The NeuN rbfox3 (Catalog #AAA8516062) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The NeuN Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NeuN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the NeuN rbfox3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NeuN, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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