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Western Blot (WB)
(Western blot analysis of NeuN on human brain lysates using anti-NeuN antibody at 1/1, 000 dilution. (NeuN is expressed as two alternate transcripts with apparent SDS-PAGE molecular weight 46 and 48kDa))
Rabbit NeuN Monoclonal Antibody | anti-NeuN antibody
NeuN Antibody
Gene Names
RBFOX3; FOX3; NEUN; FOX-3; HRNBP3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
NeuN; Monoclonal Antibody; NeuN Antibody; FLJ56884 antibody; FLJ58356 antibody; Fox-1 homolog C antibody; fox1 homolog C antibody; Fox3 antibody; FOX3NeuN antibody; hexaribonucleotide binding protein 3 antibody; HRNBP3 antibody; NEUN antibody; neuronal nuclei antibody; Rbfox3 antibody; RFOX3_HUMAN antibody; RNA binding protein fox-1 homolog 3 antibody; RNA binding protein; fox 1 homolog (C. elegans) 3 antibody; hide; anti-NeuN antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
SR45-07
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-NeuN antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-1:2000
IHC: 1:200-1:500
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
IHC: 1:200-1:500
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of NeuN on human brain lysates using anti-NeuN antibody at 1/1, 000 dilution. (NeuN is expressed as two alternate transcripts with apparent SDS-PAGE molecular weight 46 and 48kDa))
Western Blot (WB)
(Western blot analysis of NeuN on human brain lysates using anti-NeuN antibody at 1/1, 000 dilution. (NeuN is expressed as two alternate transcripts with apparent SDS-PAGE molecular weight 46 and 48kDa))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NeuN antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NeuN antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-NeuN antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-NeuN antibody. Counter stained with hematoxylin.)
Immunocytochemistry (ICC)
(ICC staining NeuN in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining NeuN in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of SH-SY-5Y cells with NeuN antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of SH-SY-5Y cells with NeuN antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Related Product Information for anti-NeuN antibody
Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, and dentate nucleus neurons. This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS-analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator. Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development.
Product Categories/Family for anti-NeuN antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Calculated MW: 33kDa/35kDa
Observed MW: 50kDa
Observed MW: 50kDa
NCBI Official Full Name
RNA binding protein fox-1 homolog 3
NCBI Official Synonym Full Names
RNA binding protein, fox-1 homolog (C. elegans) 3
NCBI Official Symbol
RBFOX3
NCBI Official Synonym Symbols
FOX3; NEUN; FOX-3; HRNBP3
NCBI Protein Information
RNA binding protein fox-1 homolog 3; fox-1 homolog C; neuronal nuclei; hexaribonucleotide binding protein 3
UniProt Protein Name
RNA binding protein fox-1 homolog 3
UniProt Gene Name
RBFOX3
UniProt Entry Name
RFOX3_HUMAN
Uniprot Description
NeuN: RNA-binding protein that regulates alternative splicing events.
Protein type: RNA splicing; RNA processing; DNA-binding
Chromosomal Location of Human Ortholog: 17q25.3
Cellular Component: cytoplasm; perikaryon; nucleus
Molecular Function: DNA binding; RNA binding; nucleotide binding
Biological Process: RNA splicing; mRNA processing; regulation of alternative nuclear mRNA splicing, via spliceosome
Research Articles on NeuN
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Product Notes
The NeuN rbfox3 (Catalog #AAA9435479) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NeuN Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NeuN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:1000-1:2000 IHC: 1:200-1:500 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the NeuN rbfox3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NeuN, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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