Immunohistochemistry (IHC)
(Immunohistochemistry-Paraffin: Staining of Myosin heavy chain in human myocardium using MBS631517 and DAB with hematoxylin counterstain.)
Mouse Myosin, Cardiac, Heavy Chain Monoclonal Antibody
Myosin, Cardiac, Heavy Chain (Alpha MHC, ASD3, CMD1S, CMH1, MGC138376, MGC138378, MPD1, MYH6, MYH7, MYHC, MyHC-alpha, MYHCA, MyHC-beta, MYHCB, Myosin Heavy Chain Cardiac Muscle alpha Isoform, Myosin Heavy Chain Cardiac Muscle beta Isoform, Myosin Heavy Po
Reactivity
Bovine, Canine, Human, Mouse, Porcine, Rabbit, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
PurifiedPurified
Synonyms
Myosin; Cardiac; Heavy Chain; Monoclonal Antibody; Heavy Chain (Alpha MHC; ASD3; CMD1S; CMH1; MGC138376; MGC138378; MPD1; MYH6; MYH7; MYHC; MyHC-alpha; MYHCA; MyHC-beta; MYHCB; Myosin Heavy Chain Cardiac Muscle alpha Isoform; Myosin Heavy Chain Cardiac Muscle beta Isoform; Myosin Heavy Po; Anti -Myosin; anti-Myosin; Heavy Chain antibody
Host
Mouse
Reactivity
Bovine, Canine, Human, Mouse, Porcine, Rabbit, Rat
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
8L880
Specificity
Recognizes human myosin alpha and beta heavy chains. Reacts with heavy chains of human myosin isolated from
Purity/Purification
Purified
Purified
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.4. No preservatives added.
Applicable Applications for anti-Myosin, Cardiac, Heavy Chain antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.
Dilution: Immunohistochemistry (Frozen): 10ug/ml tissue treatment may reduce or destroy the myosin binding sites
Western Blot: Detects a band at ~223kD
Indirect Immunofluorescence (Frozen): Binds to A-band of thick filament of cardiac and skeletal muscle myosin and indirect immunoperoxidase staining of Carnoy fixed tissues.
Dilution: Immunohistochemistry (Frozen): 10ug/ml tissue treatment may reduce or destroy the myosin binding sites
Western Blot: Detects a band at ~223kD
Indirect Immunofluorescence (Frozen): Binds to A-band of thick filament of cardiac and skeletal muscle myosin and indirect immunoperoxidase staining of Carnoy fixed tissues.
Immunogen
Purified full length native human Myosin Cardiac, Heavy Chain.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunohistochemistry (IHC)
(Immunohistochemistry-Paraffin: Staining of Myosin heavy chain in human myocardium using MBS631517 and DAB with hematoxylin counterstain.)
Immunohistochemistry (IHC)
(Immunohistochemistry-Paraffin: Staining of Myosin heavy chain in human myocardium using MBS631517 and DAB with hematoxylin counterstain.)
Immunohistochemistry (IHC)
(Immunohistochemistry-Paraffin: Staining of Myosin heavy chain in human stomach using MBS631517 and DAB with hematoxylin counterstain.)
Immunohistochemistry (IHC)
(Immunohistochemistry-Paraffin: Staining of Myosin heavy chain in human stomach using MBS631517 and DAB with hematoxylin counterstain.)
Western Blot (WB)
(Western Blot analysis of total cardiac myosin expression in tissue lysates of rat heart (lanes 1-5), liver (lanes 6-7) and lung (lanes 8-9) using MBS631517 at 1:500. Increasing amounts of total protein were loaded as follow: lanes 1, 6 and 8: 0ug; lane 2: 50ug; lanes 3, 7 and 9: 250ug; lane 4: 400ug; lane 5: 500ug. Secondary antibody: IgG Fc (HRP) Goat anti-mouse; 1:5000)
Western Blot (WB)
(Western Blot analysis of total cardiac myosin expression in tissue lysates of rat heart (lanes 1-5), liver (lanes 6-7) and lung (lanes 8-9) using MBS631517 at 1:500. Increasing amounts of total protein were loaded as follow: lanes 1, 6 and 8: 0ug; lane 2: 50ug; lanes 3, 7 and 9: 250ug; lane 4: 400ug; lane 5: 500ug. Secondary antibody: IgG Fc (HRP) Goat anti-mouse; 1:5000)
Related Product Information for anti-Myosin, Cardiac, Heavy Chain antibody
Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Cardiac MHC exists as two isoforms in humans, alpha-cardiac MHC and beta-cardiac MHC. These two isoforms are expressed in different amounts in the human heart. During normal physiology, beta-cardiac MHC is the predominant form, with the alpha-isoform contributing around only 7% of the total MHC. Mutations of the MHC genes are associated with several different dilated and hypertrophic cardiomyopathies.
Product Categories/Family for anti-Myosin, Cardiac, Heavy Chain antibody
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Product Notes
The Myosin, Cardiac, Heavy Chain (Catalog #AAA631517) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Myosin, Cardiac, Heavy Chain (Alpha MHC, ASD3, CMD1S, CMH1, MGC138376, MGC138378, MPD1, MYH6, MYH7, MYHC, MyHC-alpha, MYHCA, MyHC-beta, MYHCB, Myosin Heavy Chain Cardiac Muscle alpha Isoform, Myosin Heavy Chain Cardiac Muscle beta Isoform, Myosin Heavy Po reacts with Bovine, Canine, Human, Mouse, Porcine, Rabbit, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Myosin, Cardiac, Heavy Chain can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Dilution: Immunohistochemistry (Frozen): 10ug/ml tissue treatment may reduce or destroy the myosin binding sites Western Blot: Detects a band at ~223kD Indirect Immunofluorescence (Frozen): Binds to A-band of thick filament of cardiac and skeletal muscle myosin and indirect immunoperoxidase staining of Carnoy fixed tissues. Researchers should empirically determine the suitability of the Myosin, Cardiac, Heavy Chain for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myosin, Cardiac, Heavy Chain, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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