Rabbit anti-Human, Mouse MYH7 Polyclonal Antibody | anti-MYH7 antibody

MYH7, NT (MYH7, MYHCB, Myosin-7, Myosin heavy chain 7, Myosin heavy chain slow isoform, Myosin heavy chain, cardiac muscle beta isoform) (FITC)

Cat. Num. AAA6323278

• Gene Names: MYH7; CMH1; MPD1; SPMD; SPMM; CMD1S; MYHCB
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MYH7; Polyclonal Antibody; NT (MYH7; MYHCB; Myosin-7; Myosin heavy chain 7; Myosin heavy chain slow isoform; Myosin heavy chain; cardiac muscle beta isoform) (FITC); cardiac muscle beta isoform; anti-MYH7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-MYH7 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MYH7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 184-211 amino acids from the N-terminal region of human MYH7.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MYH7 Antibody (N-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the MYH7 antibody detected the MYH7 protein (arrow).)

Related Product Information for anti-MYH7 antibody

Muscle myosin is a hexameric protein containing 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, myosin storage myopathy, dilated cardiomyopathy, and Laing early-onset distal myopathy.

Product Categories/Family for anti-MYH7 antibody

Antibodies; Cardiac Markers

References

Millat, G., et al. Clin. Chim. Acta 411 (23-24), 1983-1991 (2010): Eijgelsheim, M., et al. Hum. Mol. Genet. 19(19):3885-3894(2010). Millat, G., et al. Eur J Med Genet 53(5):261-267(2010). Muelas, N., et al. Neurology 75(8):732-741(2010). Zheng, D.D., et al. J. Int. Med. Res. 38(3):810-820(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 115496169
• NCBI GeneID: 4625
• NCBI Accession #: NP_000248.2
• NCBI GenBank Nucleotide #: NM_000257.3
• UniProt Accession #: P12883; Q14836; Q14837; Q14904; Q16579; Q2M1Y6; Q92679; Q9H1D5; Q9UDA2; Q9UMM8; A2TDB6; B6D424
• Molecular Weight: 223,097 Da
• NCBI Official Full Name: myosin-7
• NCBI Official Synonym Full Names: myosin heavy chain 7
• NCBI Official Symbol: MYH7
• NCBI Official Synonym Symbols: CMH1; MPD1; SPMD; SPMM; CMD1S; MYHCB
• NCBI Protein Information: myosin-7
• UniProt Protein Name: Myosin-7
• Protein Family: Myosin
• UniProt Gene Name: MYH7
• UniProt Synonym Gene Names: MYHCB; MyHC-slow; MyHC-beta
• UniProt Entry Name: MYH7_HUMAN

NCBI Description

Muscle myosin is a hexameric protein containing 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, myosin storage myopathy, dilated cardiomyopathy, and Laing early-onset distal myopathy. [provided by RefSeq, Jul 2008]

Uniprot Description

MYH7: Muscle contraction. Defects in MYH7 are the cause of familial hypertrophic cardiomyopathy type 1 (CMH1). Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Defects in MYH7 are the cause of myopathy myosin storage (MYOMS). In this disorder, muscle biopsy shows type 1 fiber predominance and increased interstitial fat and connective tissue. Inclusion bodies consisting of the beta cardiac myosin heavy chain are present in the majority of type 1 fibers, but not in type 2 fibers. Defects in MYH7 are the cause of scapuloperoneal myopathy MYH7-related (SPMM); also known as scapuloperoneal syndrome myopathic type. SPMM is a progressive muscular atrophia beginning in the lower legs and affecting the shoulder region earlier and more severely than distal arm. Defects in MYH7 are a cause of cardiomyopathy dilated type 1S (CMD1S). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Defects in MYH7 are the cause of myopathy distal type 1 (MPD1). MPD1 is a muscular disorder characterized by early-onset selective weakness of the great toe and ankle dorsiflexors, followed by weakness of the finger extensors. Mild proximal weakness occasionally develops years later after the onset of the disease.

Protein type: Motor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: nucleoplasm; sarcomere; focal adhesion; cytoplasm; stress fiber; muscle myosin complex; Z disc; myosin complex

Molecular Function: calmodulin binding; microfilament motor activity; protein binding; ATPase activity; actin-dependent ATPase activity; actin binding; ATP binding

Biological Process: adult heart development; striated muscle contraction; muscle contraction; regulation of heart rate; metabolic process; ventricular cardiac muscle morphogenesis; regulation of the force of heart contraction; muscle filament sliding

Disease: Myopathy, Distal, 1; Scapuloperoneal Myopathy, Myh7-related; Myopathy, Myosin Storage; Cardiomyopathy, Dilated, 1s; Myopathy, Congenital, With Fiber-type Disproportion; Cardiomyopathy, Familial Hypertrophic, 1; Myopathy, Myosin Storage, Autosomal Recessive