Human IL-16 (Interleukin 16) CLIA Kit | IL-16 clia kit
Human IL-16 (Interleukin 16) CLIA Kit
Reactivity
Human
Synonyms
IL-16 (Interleukin 16); Human IL-16 (Interleukin 16) CLIA Kit; IL16; LCF; NIL16; PRIL16; prIL-16; IL-16 clia kit
Reactivity
Human
Specificity
This kit recognizes natural and recombinant IL-16. No significant cross-reactivity or interference between IL-16 and analogues was observed.
Sequence Length
130
Assay Type
Sandwich
Detection Range
12.5-800pg/mL
Sensitivity
Min: 7.5pg/mL; Max: 800pg/mL
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IL-16 clia kit
Intended Uses: This CLIA kit applies to the in vitro quantitative determination of IL-16 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to IL-16. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-16 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-16, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of IL-16. You can calculate the concentration of IL-16 in the samples by comparing the RLU value of the samples to the standard curve.
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to IL-16. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-16 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-16, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of IL-16. You can calculate the concentration of IL-16 in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
59,345 Da
NCBI Official Full Name
interleukin-16
UniProt Protein Name
Pro-interleukin-16
UniProt Gene Name
IL16
UniProt Synonym Gene Names
IL-16; LCF
UniProt Entry Name
IL16_HUMAN
Uniprot Description
IL16: Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4. 3 isoforms of the human protein are produced by alternative promoter.
Protein type: Motility/polarity/chemotaxis; DNA-binding; Cytokine
Chromosomal Location of Human Ortholog: 15q26.3
Cellular Component: extracellular space; cytoplasm; plasma membrane; nucleus
Molecular Function: cytokine activity
Biological Process: transcription, DNA-dependent; viral reproduction; regulation of transcription, DNA-dependent; leukocyte chemotaxis; immune response; induction of positive chemotaxis
