Mouse Cardiac Myosin Heavy Chain Monoclonal Antibody | anti-CMHC antibody

Cardiac Myosin Heavy Chain Mouse Monoclonal (3-48_G5C7)

Cat. Num. AAA588374

• Gene Names: MYH7; CMH1; MPD1; SPMD; SPMM; CMD1S; MYHCB
• Reactivity: Human, Rat, Mouse; Predicted: Canine, Bovine and Rabbit
• Applications: ELISA, Western Blot, Immunofluorescence, Immunohistochemistry
• Synonyms: Cardiac Myosin Heavy Chain; Monoclonal Antibody; Cardiac Myosin Heavy Chain Mouse Monoclonal (3-48_G5C7); anti-CMHC antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse; Predicted: Canine, Bovine and Rabbit
• Clonality: Monoclonal
• Isotype: IgG1 kappa
• Clone Number: 3-48
• Specificity: This antibody recognizes alpha and beta type heavy chains of light meromyosin fragments (LMM) of cardiac myosin heavy chains. It also reacts with LMM fragments of cardiac myosin and beta heavy chain of slow human skeletal muscle. The antibody does not crossreact with human myosin light chains.
• Form/Format: Lyophilized protein G purified in PBS pH7.4 (Full IgG)
• Sequence Length: 1934

• Applicable Applications for anti-CMHC antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
• Application Notes: If diluted in 100muL: WB: 1:500; IHC: 1:300.
• Preservative: None
• Preparation and Storage: Lyophilized antibody can be kept at 4 degree C for up to 3 months and should be kept at-20 degree C for long-term storage.; To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20 degree C) or for long-term storage (-80 degree C).; For maximum recovery of product centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Formaldehyde-fixed paraffin-embedded (FFPE) Tissue Slides. Human heart ventricle following immunostaining with cMHC 3-4B and irrelevant NeuAc monoClonal antibody at low microscopical magnification. A: cMHC 3-48-labeled cardiomyocytes. FFPE section of human heart ventricle was submitted to HIAR condition with citrate buffer prior to immunostaining. Immunostaining was revealed following incubation with biotinylated goat anti-mouse IgG, 20 minutes at 20C and horse radish peroxidase-diaminobemzidine enzymatic reaction causing brown precipitate to appear atthe sites of primary antibody binding. B: Control immunostaining performed following HIAR condition on the same microscopical slide with NeuAc monoClonal antibody showing no immunostaining but presence of light background, emphasizing specificity of immunostaining in (A). Magnification x30 C: Fragment of the same slide as in (A) with heart ventricle cardiomyocytes immunostained (brown). Magnification x580)

Testing Data

Related Product Information for anti-CMHC antibody

Myosin is a highly conserved, ubiquitously expressed protein that hydrolyzes ATR and this reaction provides the energy required for muscle contraction that interacts with actin to generate the force for cellular movements. It is a hexameric protein composed of four light chains and two heavy chains, each containing an actin-binding site and an ATP hydrolysis site. The heavy chains are encoded by the MYH gene family and were first isolated from a human fetal skeletal muscle and are the major determinant in the speed of contraction of skeletal muscle. Cardiac myosin exists as two isoforms in humans, Alpha-cardiac myosins and beta-cardiac myosins. These two isoforms are expressed in different amounts in the human heart. Beta-cardiac myosin s the predominant form during normal physiology while the alpha-isoform contributes for approximately 7% of the total myosin. Mutations of the MYH genes are associated with several different dilated and hypertrophic cardiomyopathies.

NCBI and Uniprot Product Information

• NCBI GI #: 29727
• NCBI GeneID: 4625
• UniProt Accession #: P12883; Q14836; Q14837; Q14904; Q16579; Q2M1Y6; Q92679; Q9H1D5; Q9UDA2; Q9UMM8; A2TDB6; B6D424
• Molecular Weight: 223,097 Da
• NCBI Official Full Name: cardiac beta myosin heavy chain
• NCBI Official Synonym Full Names: myosin heavy chain 7
• NCBI Official Symbol: MYH7
• NCBI Official Synonym Symbols: CMH1; MPD1; SPMD; SPMM; CMD1S; MYHCB
• NCBI Protein Information: myosin-7
• UniProt Protein Name: Myosin-7
• UniProt Gene Name: MYH7
• UniProt Synonym Gene Names: MYHCB; MyHC-slow; MyHC-beta

NCBI Description

Muscle myosin is a hexameric protein containing 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, myosin storage myopathy, dilated cardiomyopathy, and Laing early-onset distal myopathy. [provided by RefSeq, Jul 2008]

Uniprot Description

Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Forms regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle.