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Immunohistochemistry (IHC) (Immunohistochemistry with rat sensory ganglia tissue)

Rabbit TRPA1 Polyclonal Antibody | anti-TRPA1 antibody

TRPA1 antibody - middle region

Gene Names
TRPA1; FEPS; FEPS1; ANKTM1
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast
Applications
Immunohistochemistry, Western Blot, Immunofluorescence
Purity
Affinity Purified
Synonyms
TRPA1; Polyclonal Antibody; TRPA1 antibody - middle region; anti-TRPA1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Sequence
Synthetic peptide located within the following region: KCTDRLDEDGNTALHFAAREGHAKAVALLLSHNADIVLNKQQASFLHLAL
Sequence Length
1119
Applicable Applications for anti-TRPA1 antibody
Immunohistochemistry (IHC), Western Blot (WB), Immunofluorescence (IF)
Homology
Cow: 93%; Dog: 90%; Guinea Pig: 93%; Horse: 86%; Human: 100%; Mouse: 100%; Rabbit: 93%; Rat: 100%; Yeast: 100%
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human TRPA1
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry with rat sensory ganglia tissue)

Immunohistochemistry (IHC) (Immunohistochemistry with rat sensory ganglia tissue)

Immunohistochemistry (IHC)

(Sample Type :Section of TRPM8-GFP transgenic mouse trigeminal gangliaPrimary Antibody Dilution :1:250Secondary Antibody :Goat Anti-Rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:1000Color/Signal Descriptions :TRPA1: Red TRPM8-GFP:: GreenGene Name :TRPA1Submitted by :Dr. Daniel McCoy, USC)

Immunohistochemistry (IHC) (Sample Type :Section of TRPM8-GFP transgenic mouse trigeminal gangliaPrimary Antibody Dilution :1:250Secondary Antibody :Goat Anti-Rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:1000Color/Signal Descriptions :TRPA1: Red TRPM8-GFP:: GreenGene Name :TRPA1Submitted by :Dr. Daniel McCoy, USC)

Western Blot (WB)

(Host: RabbitTarget Name: TRPA1Sample Tissue: Human HepG2Antibody Dilution: 1.0ug/ml)

Western Blot (WB) (Host: RabbitTarget Name: TRPA1Sample Tissue: Human HepG2Antibody Dilution: 1.0ug/ml)

Western Blot (WB)

(Host: RabbitTarget Name: TRPA1Sample Tissue: Human THP-1 Whole CellAntibody Dilution: 1ug/ml)

Western Blot (WB) (Host: RabbitTarget Name: TRPA1Sample Tissue: Human THP-1 Whole CellAntibody Dilution: 1ug/ml)

Western Blot (WB)

(WB Suggested Anti-TRPA1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Stomach)

Western Blot (WB) (WB Suggested Anti-TRPA1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Stomach)
Related Product Information for anti-TRPA1 antibody
This is a rabbit polyclonal antibody against TRPA1. It was validated on Western Blot using a cell lysate as a positive control.

Target Description: The structure of the protein encoded by this gene is highly related to both the protein ankyrin and transmembrane proteins. The specific function of this protein has not yet been determined; however, studies indicate the function may involve a role in sig
Product Categories/Family for anti-TRPA1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
127kDa
NCBI Official Full Name
transient receptor potential cation channel subfamily A member 1
NCBI Official Synonym Full Names
transient receptor potential cation channel subfamily A member 1
NCBI Official Symbol
TRPA1
NCBI Official Synonym Symbols
FEPS; FEPS1; ANKTM1
NCBI Protein Information
transient receptor potential cation channel subfamily A member 1
UniProt Protein Name
Transient receptor potential cation channel subfamily A member 1
UniProt Gene Name
TRPA1
UniProt Synonym Gene Names
ANKTM1
UniProt Entry Name
TRPA1_HUMAN

NCBI Description

The structure of the protein encoded by this gene is highly related to both the protein ankyrin and transmembrane proteins. The specific function of this protein has not yet been determined; however, studies indicate the function may involve a role in signal transduction and growth control. [provided by RefSeq, Jul 2008]

Uniprot Description

TRPA1: Receptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. Acts also as a ionotropic cannabinoid receptor by being activated by delta(9)- tetrahydrocannabinol (THC), the psychoactive component of marijuana. Not involved in menthol sensation. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system. Belongs to the transient receptor (TC 1.A.4) family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 8q13

Cellular Component: stereocilium bundle; integral to plasma membrane; plasma membrane

Molecular Function: channel activity; calcium channel activity

Biological Process: response to drug; detection of mechanical stimulus involved in sensory perception of pain; detection of chemical stimulus involved in sensory perception of pain; response to hydrogen peroxide; response to pain; ion transport; thermoception; response to cold; transmembrane transport

Disease: Episodic Pain Syndrome, Familial, 1

Research Articles on TRPA1

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Product Notes

The TRPA1 trpa1 (Catalog #AAA3202474) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRPA1 antibody - middle region reacts with Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast and may cross-react with other species as described in the data sheet. AAA Biotech's TRPA1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB), Immunofluorescence (IF). Researchers should empirically determine the suitability of the TRPA1 trpa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: KCTDRLDEDG NTALHFAARE GHAKAVALLL SHNADIVLNK QQASFLHLAL. It is sometimes possible for the material contained within the vial of "TRPA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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