Rat anti-Mouse MBL-C Monoclonal Antibody | anti-Mbl2 antibody

MBL-C (Mannose-binding Protein C, Mannan-binding Protein, RA-reactive Factor P28A Subunit, RARF/P28A, Mbl2)

Cat. Num. AAA6008828

• Gene Names: Mbl2; MBL; L-MBP; MBL-C; MBP-C
• Reactivity: Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: MBL-C; Monoclonal Antibody; MBL-C (Mannose-binding Protein C; Mannan-binding Protein; RA-reactive Factor P28A Subunit; RARF/P28A; Mbl2); Anti -MBL-C (Mannose-binding Protein C; anti-Mbl2 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 13A53
• Specificity: Recognizes mouse MBL-C.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

• Applicable Applications for anti-Mbl2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, ELISA, Immunofluorescence and Immunohistochemistry.; Dilution: ELISA: 1ug/ml TBS/Tween-20/Ca; Immunofluorescence: Hyphae of C. albicans were fixed on poly-l-lysin coated slides with ice-cold aceton. Slides were incubated for 1h at RT; Immunohistochemistry (frozen): (7um) of mouse tissue were acetone fixed and stained o/n at 4 degree C in TBS/2% goat serum; Western Blot: On a 12% non-reducing SDS-PAGE a prominent band of ca. 50kD is detected. Under reducing conditions a 26kD band is detected
• Immunogen: Purified MBL-C emulsified in complete Freund's adjuvant
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(MBL-C deposition in developing murine atherosclerotic lesions following 10 weeks of high fat feeding. MBL-C was detected in and around invading macrophages invading the intima (insert). MBL-C bound, similar to MBL-A, at sites of necrosis (upper right corner). No MBL-C binding was shown in the media or on fibrous caps covering the thickened intima.)

Related Product Information for anti-Mbl2 antibody

Mannose binding lectin (MBL), also called mannose- or mannan-binding protein (MBP), is a member of the group of collectins. MBL is an important pattern-recognition receptor in the innate immune system. The protein mediates innate immune responses, such as activation of the complement lectin pathway and phagocytosis, to help fight infections. MBL is an oligomeric lectin that recognizes carbohydrates as mannose and N-acetylglucosamine on pathogens. MBL contains a cysteine rich, a collagen like and a carbohydrate recognition domain. Binding of MBL leads to the activation of MBL- associated serine proteases (MASP's). Activated MASP-2 cleaves C4 and C2 in a similar way as C1s do for the classical pathway (CP) leading to the formation of C4b2a, cleavage of the classical pathway convertase C3, and eventually complement activation up to the formation of the membrane attack complex. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways. MBL is predominantly synthesized by hepatocytes and has been isolated from the liver or serum of several vertebrate species. Only one form of human MBL has been characterized, while two forms are found in rhesus monkeys, rabbits, rats and mice. The mouse forms are known as MBL-A and MBL-C. The MBL-C concentrations in serum are about 6-fold compared to that of MBL-A. MBL-A, but not MBL-C, was found to be an acute phase protein in casein and LPS-injection models. MBL-C exists in higher oligomeric forms than MBL-A. This monoclonal antibody is a calcium-dependent antibody.

Product Categories/Family for anti-Mbl2 antibody

Antibodies; Abs to Lectins

NCBI and Uniprot Product Information

• NCBI GI #: 6754656
• NCBI GeneID: 17195
• NCBI Accession #: NP_034906.1
• NCBI GenBank Nucleotide #: NM_010776.1
• UniProt Accession #: P41317
• Molecular Weight: 25,957 Da
• NCBI Official Full Name: mannose-binding protein C
• NCBI Official Synonym Full Names: mannose-binding lectin (protein C) 2
• NCBI Official Symbol: Mbl2
• NCBI Official Synonym Symbols: MBL; L-MBP; MBL-C; MBP-C
• NCBI Protein Information: mannose-binding protein C; RARF/P28A; mannan-binding protein; mannose binding lectin (C); RA-reactive factor P28A subunit; mannose binding lectin, liver (C)
• UniProt Protein Name: Mannose-binding protein C
• Protein Family: Mannose-binding protein
• UniProt Gene Name: Mbl2
• UniProt Synonym Gene Names: MBP-C; RARF/P28A
• UniProt Entry Name: MBL2_MOUSE

Uniprot Description

MBL2: Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; collagen; extracellular region

Molecular Function: mannose binding; protease binding; protein self-association; galactose binding; calcium ion binding; carbohydrate binding; calcium-dependent protein binding; receptor binding

Biological Process: defense response to Gram-positive bacterium; killing by host of symbiont cells; immune system process; positive regulation of phagocytosis; innate immune response; complement activation, lectin pathway; complement activation, classical pathway; negative regulation of viral reproduction