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Quality Control (Western blot analysis of immunized recombinant protein, using anti-RHOB monoclonal antibody. )

Mouse anti-Human RHOB Monoclonal Antibody | anti-RHOB antibody

Mouse monoclonal antibody Anti-Human RHOB

Gene Names
RHOB; ARH6; ARHB; RHOH6; MST081; MSTP081
Reactivity
Human
Applications
Dot Blot
Synonyms
RHOB; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human RHOB; Homo sapiens ras homolog gene family; member B (RHOB); ARH6;ARHB; RHOH6; MST081; MSTP081; anti-RHOB antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2916C2a
Concentration
100 ug/ml (1.0 ml) (varies by lot)
Sequence Length
2387
Applicable Applications for anti-RHOB antibody
Dot Blot (DB)
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.

Quality Control

(Western blot analysis of immunized recombinant protein, using anti-RHOB monoclonal antibody. )

Quality Control (Western blot analysis of immunized recombinant protein, using anti-RHOB monoclonal antibody. )

Quality Control #2

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Related Product Information for anti-RHOB antibody
Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. [UniProtKB Function]

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
388
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,123 Da
NCBI Official Full Name
Homo sapiens ras homolog family member B (RHOB), mRNA
NCBI Official Synonym Full Names
ras homolog family member B
NCBI Official Symbol
RHOB
NCBI Official Synonym Symbols
ARH6; ARHB; RHOH6; MST081; MSTP081
NCBI Protein Information
rho-related GTP-binding protein RhoB; Aplysia RAS-related homolog 6; h6; oncogene RHO H6; ras homolog gene family, member B; rho cDNA clone 6
UniProt Gene Name
RHOB
UniProt Entry Name
RHOB_HUMAN

Uniprot Description

RHOB: a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Controls the reorganization of actins into podosomes. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders.

Protein type: G protein, monomeric, Rho; Tumor suppressor; G protein, monomeric; Motility/polarity/chemotaxis; G protein

Chromosomal Location of Human Ortholog: 2p24

Cellular Component: focal adhesion; late endosome membrane; early endosome; plasma membrane; endosome membrane; nucleus; cytosol; cleavage furrow

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding

Biological Process: platelet activation; axon guidance; transformed cell apoptosis; apoptosis; positive regulation of apoptosis; metabolic process; cytokinesis; Rho protein signal transduction; negative regulation of cell cycle; intracellular protein transport; regulation of small GTPase mediated signal transduction; positive regulation of angiogenesis; small GTPase mediated signal transduction; endosome to lysosome transport; angiogenesis; cell adhesion; blood coagulation

Research Articles on RHOB

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Product Notes

The RHOB rhob (Catalog #AAA120245) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse monoclonal antibody Anti-Human RHOB reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RHOB can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB). Researchers should empirically determine the suitability of the RHOB rhob for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RHOB, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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