Mannan Binding Lectin/MBL2 Recombinant Protein | MBL2 recombinant protein

Mannan Binding Lectin/MBL2 Protein, Mouse, Recombinant (mFc Tag)

Cat. Num. AAA8122508

• Gene Names: Mbl2; MBL; L-MBP; MBL-C; MBP-C
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Mannan Binding Lectin/MBL2; Mannan Binding Lectin/MBL2 Protein; Mouse; Recombinant (mFc Tag); Mouse MBL-2/MBL Protein (Fc Tag); L-MBP Protein; MBL Protein; MBL-C Protein; MBP-C Protein; mannose-binding lectin (protein C) 2; soluble; MBL2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Glu19-Asp244

• Species: Mouse
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Asp
• Tag: N-mouse IgG1-Fc
• Protein Construction: A DNA sequence encoding the mouse Mbl2 (NP_034906.1)(Glu19-Asp244) was expressed with the Fc region of mouse IgG1 at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for MBL2 recombinant protein

Background: MBL (mannose-binding lectin) is primarily a liver-derived collagen-like serum protein, which binds sugar structures on micro-organisms and dying host cells and is one of the four known mediators that initiate activation of the complement system via the lectin pathway. MBL and the ficolins (Ficolin-1, Ficolin-2, and Ficolin-3) are soluble collagen-like proteins that are involved in innate immune defense. They bind sugar structures or acetylated compounds present on microorganisms and dying host cells and they initiate activation of the lectin complement pathway in varying degrees. MBL2 encodes the mannose-binding lectin, which is a key player in the innate immune system and has recently been found to play a role in the development of type 1 diabetes and gestational diabetes mellitus. Common variant alleles situated both in the promoter and structural regions of the MBL2 gene influence the stability and the serum concentration of the protein. Several polymorphisms in the promoter and structural regions of MBL2 adversely affect the plasma concentration and the oligomeric state of MBL. The possession of mutant alleles has been linked to disease outcomes for a variety of bacterial and viral infections. Mutant MBL2 haplotypes have been linked to disease progression and response to therapy in HCV infection.

Product Categories/Family for MBL2 recombinant protein

Complement Activation Pathways

References

Garred P, et al. (2006) Mannose-binding lectin and its genetic variants. Genes Immun. 7(2): 85-94.Brown KS, et al. (2007) Mannan binding lectin and viral hepatitis. Immunol Lett. 108(1): 34-44.Garred P. (2008) Mannose-binding lectin genetics: from A to Z. Biochem Soc Trans. 36(Pt 6): 1461-6.Garred P, et al. (2009) MBL2, FCN1, FCN2 and FCN3-The genes behind the initiation of the lectin pathway of complement. Mol Immunol. 46(14): 2737-44.Muller YL, et al. (2010) Functional Variants in MBL2 Are Associated With Type 2 Diabetes and Pre-Diabetes Traits in Pima Indians and the Old Order Amish. Diabetes. 59(8): 2080-5.

NCBI and Uniprot Product Information

• NCBI GI #: 6754656
• NCBI GeneID: 17195
• NCBI Accession #: NP_034906.1
• NCBI GenBank Nucleotide #: NM_010776.1
• UniProt Accession #: P41317
• Molecular Weight: 25,957 Da
• NCBI Official Full Name: mannose-binding protein C
• NCBI Official Synonym Full Names: mannose-binding lectin (protein C) 2
• NCBI Official Symbol: Mbl2
• NCBI Official Synonym Symbols: MBL; L-MBP; MBL-C; MBP-C
• NCBI Protein Information: mannose-binding protein C; RARF/P28A; mannan-binding protein; mannose binding lectin (C); RA-reactive factor P28A subunit; mannose binding lectin, liver (C)
• UniProt Protein Name: Mannose-binding protein C
• Protein Family: Mannose-binding protein
• UniProt Gene Name: Mbl2
• UniProt Synonym Gene Names: MBP-C; RARF/P28A
• UniProt Entry Name: MBL2_MOUSE

Uniprot Description

MBL2: Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; collagen; extracellular region

Molecular Function: mannose binding; protein self-association; protease binding; galactose binding; calcium ion binding; carbohydrate binding; calcium-dependent protein binding; receptor binding

Biological Process: defense response to Gram-positive bacterium; killing by host of symbiont cells; immune system process; positive regulation of phagocytosis; innate immune response; complement activation, lectin pathway; complement activation, classical pathway; negative regulation of viral reproduction