MBL-2 / MBL recombinant protein

Mouse MBL-2 / MBL Protein (His Tag)

Cat. Num. AAA2547244

• Gene Names: Mbl2; MBL; L-MBP; MBL-C; MBP-C
• Purity: > 90 % as determined by SDS-PAGE.
• Synonyms: MBL-2 / MBL; Mouse MBL-2 / MBL Protein (His Tag); MBL-2; MBL; MBL-2 / MBL recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 244

• Application Notes: The recombinant mouse Mbl2 consists 245 amino acids and predicts a molecular mass of 26.3 kDa.
• Predicted N Terminal: His
• Endotoxin: <1.0 EU per mug protein as determined by the LAL method.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for MBL-2 / MBL recombinant protein

Background: MBL (mannose-binding lectin) is primarily a liver-derived collagen-like serum protein, which binds sugar structures on micro-organisms and on dying host cells and is one of the four known mediators that initiate activation of the complement system via the lectin pathway. MBL and the ficolins (Ficolin-1, Ficolin-2 and Ficolin-3) are soluble collagen-like proteins that are involved in innate immune defence. They bind sugar structures or acetylated compounds present on microorganisms and on dying host cells and they initiate activation of the lectin complement pathway in varying degrees. MBL2 encodes the mannose-binding lectin, which is a key player in the innate immune system and has recently been found to play a role in development of type 1 diabetes and gestational diabetes mellitus. Common variant alleles situated both in promoter and structural regions of the MBL2 gene influence the stability and the serum concentration of the protein. Several polymorphisms in the promoter and structural regions of MBL2 adversely affect the plasma concentration and oligomeric state of MBL. The possession of mutant alleles has been linked to disease outcome for a variety of bacterial and viral infections. Mutant MBL2 haplotype s have been linked to disease progression and response to therapy in HCV infection.

Description: A DNA sequence encoding the mouse Mbl2 (NP_034906.1) (Glu19-Asp244) was expressed with a polyhistidine tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 6754656
• NCBI GeneID: 17195
• NCBI Accession #: NP_034906.1
• NCBI GenBank Nucleotide #: NM_010776.1
• UniProt Accession #: P41317
• Molecular Weight: 25,957 Da
• NCBI Official Full Name: mannose-binding protein C
• NCBI Official Synonym Full Names: mannose-binding lectin (protein C) 2
• NCBI Official Symbol: Mbl2
• NCBI Official Synonym Symbols: MBL; L-MBP; MBL-C; MBP-C
• NCBI Protein Information: mannose-binding protein C
• UniProt Protein Name: Mannose-binding protein C
• UniProt Gene Name: Mbl2
• UniProt Synonym Gene Names: MBP-C; RARF/P28A

Uniprot Description

MBL2: Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19 C1|19 25.14 cM

Cellular Component: extracellular space

Molecular Function: calcium ion binding; calcium-dependent protein binding; galactose binding; mannose binding; protease binding; protein self-association; receptor binding

Biological Process: complement activation, lectin pathway; defense response to Gram-positive bacterium; innate immune response; killing by host of symbiont cells; negative regulation of viral reproduction; positive regulation of phagocytosis